Forward genetic screens in model organisms have provided important insights into numerous
aspects of development, physiology and pathology. With the availability of complete …

Animal cell cytokinesis results from patterned activation of the small GTPase Rho, which
directs assembly of actomyosin in the equatorial cortex. Cytokinesis is restricted to a portion of …

In animal cells, formation of the cytokinetic furrow requires activation of the GTPase RhoA
by the guanine nucleotide exchange factor Ect2. How Ect2, which is associated with the …

At the end of cell division, cytokinesis splits the cytoplasm of nascent daughter cells and
partitions segregated sister genomes 1 , 2 . To coordinate cell division with chromosome …

Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular
transport. However, trajectory analysis procedures to infer complex transport dynamics …

Understanding the basis for cellular growth, proliferation, and function requires determining
the roles of essential genes in diverse cellular processes, including visualizing their …

Cytokinesis, the final step of cell division, begins with the formation of a cleavage furrow.
How the mitotic spindle specifies the furrow at the equator in animal cells remains unknown. …

Centromeres provide a robust model for epigenetic inheritance as they are specified by
sequence-independent mechanisms involving the histone H3-variant centromere protein A (…

The key player in directing proper chromosome segregation is the macromolecular
kinetochore complex, which mediates DNA–microtubule interactions. Previous studies testing …

10.7554/eLife.26866.001 Chromosome segregation requires robust interactions between
the macromolecular kinetochore structure and dynamic microtubule polymers. A key …