[HTML][HTML] Construct design for CRISPR/Cas-based genome editing in plants
CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
Hyperphosphorylation of CDH1 in Glioblastoma Cancer Stem Cells Attenuates APC/CCDH1 Activity and Pharmacologic Inhibition of APC/CCDH1/CDC20 …
K De, TM Grubb, AA Zalenski, KE Pfaff, D Pal… - Molecular Cancer …, 2019 - AACR
Our findings demonstrate how the activity of the APC/C CDH1 tumor suppressor is reduced
in CSCs and also validates small-molecule inhibition of the APC/C as a promising …
in CSCs and also validates small-molecule inhibition of the APC/C as a promising …
[HTML][HTML] Split selectable marker systems utilizing inteins facilitate gene stacking in plants
The ability to stack multiple genes in plants is of great importance in the development of crops
with desirable traits but can be challenging due to limited selectable marker options. Here …
with desirable traits but can be challenging due to limited selectable marker options. Here …
[HTML][HTML] In favor of establishment: regulation of chromatid cohesion in plants
P Bolaños-Villegas, K De, M Pradillo, D Liu… - Frontiers in Plant …, 2017 - frontiersin.org
In eukaryotic organisms, the correct regulation of sister chromatid cohesion, whereby sister
chromatids are paired and held together, is essential for accurate segregation of the sister …
chromatids are paired and held together, is essential for accurate segregation of the sister …
[HTML][HTML] Arabidopsis thaliana WAPL Is Essential for the Prophase Removal of Cohesin during Meiosis
K De, L Sterle, L Krueger, X Yang, CA Makaroff - PLoS genetics, 2014 - journals.plos.org
Sister chromatid cohesion, which is mediated by the cohesin complex, is essential for the
proper segregation of chromosomes in mitosis and meiosis. The establishment of stable sister …
proper segregation of chromosomes in mitosis and meiosis. The establishment of stable sister …
[HTML][HTML] Chk1-mediated phosphorylation of Cdh1 promotes the SCFβTRCP-dependent degradation of Cdh1 during S-phase and efficient cell-cycle progression
…, BR Stromberg, AL Messina, AS Dickson, K De… - Cell Death & …, 2020 - nature.com
APC/C Cdh1 is a ubiquitin ligase with roles in numerous diverse processes, including
control of cellular proliferation and multiple aspects of the DNA damage response. Precise …
control of cellular proliferation and multiple aspects of the DNA damage response. Precise …
[PDF][PDF] Mutating novel interaction sites in NRP1 reduces SARS-CoV-2 spike protein internalization
The global pandemic of coronavirus disease 2019 caused by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) virus has become a severe global health problem …
syndrome coronavirus 2 (SARS-CoV-2) virus has become a severe global health problem …
An intein-mediated split–nCas9 system for base editing in plants
Virus-assisted delivery of the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
(Cas) system represents a promising approach for editing plant …
(Cas) system represents a promising approach for editing plant …
[HTML][HTML] Core cysteine residues in the Plasminogen-Apple-Nematode (PAN) domain are critical for HGF/c-MET signaling
The Plasminogen-Apple-Nematode (PAN) domain, with a core of four to six cysteine residues,
is found in > 28,000 proteins across 959 genera. Still, its role in protein function is not fully …
is found in > 28,000 proteins across 959 genera. Still, its role in protein function is not fully …
[HTML][HTML] An interphase pool of KIF11 localizes at the basal bodies of primary cilia and a reduction in KIF11 expression alters cilia dynamics
AA Zalenski, S Majumder, K De, M Venere - Scientific reports, 2020 - nature.com
KIF11 is a homotetrameric kinesin that peaks in protein expression during mitosis. It is a
known mitotic regulator, and it is well-described that KIF11 is necessary for the formation and …
known mitotic regulator, and it is well-described that KIF11 is necessary for the formation and …