Genome remodeling in ciliated protozoa

CL Jahn, LA Klobutcher - Annual Reviews in Microbiology, 2002 - annualreviews.org
▪ Abstract The germline genomes of ciliated protozoa are dynamic structures, undergoing
massive DNA rearrangement during the formation of a functional macronucleus. Macronuclear …

All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3'terminus.

LA Klobutcher, MT Swanton… - Proceedings of the …, 1981 - National Acad Sciences
In hypotrichous ciliates, all of the macronuclear DNA is in the form of low molecular weight
molecules with an average size of approximately 2200 base pairs. Total macronuclear DNA …

[HTML][HTML] Shifty ciliates: frequent programmed translational frameshifting in euplotids

LA Klobutcher, PJ Farabaugh - Cell, 2002 - cell.com
… aediculatus La motif protein mRNA in the vicinity of the frameshift site is shown, along
with the predicted translation products from the 0 and +1 frame ORFs. The AAA-UAA-A motif …

Genetic code supports targeted insertion of two amino acids by one codon

…, DE Fomenko, HG Morrison, ML Sogin, LA Klobutcher… - Science, 2009 - science.org
Strict one-to-one correspondence between codons and amino acids is thought to be an
essential feature of the genetic code. However, we report that one codon can code for two …

The Bacillus subtilis spore coat provides “eat resistance” during phagocytic predation by the protozoan Tetrahymena thermophila

LA Klobutcher, K Ragkousi… - Proceedings of the …, 2006 - National Acad Sciences
Bacillus spores are highly resistant to many environmental stresses, owing in part to the
presence of multiple “extracellular” layers. Although the role of some of these extracellular layers …

[HTML][HTML] Genetic tool development in marine protists: emerging model organisms for experimental cell biology

…, E Kazana, PJ Keeling, N King, LA Klobutcher… - Nature …, 2020 - nature.com
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many
unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic …

Consensus inverted terminal repeat sequence of Paramecium lESs: resemblance to termini of Tc1 -related and Euplotes Tec …

LA Klobutcher, G Herrick - Nucleic acids research, 1995 - academic.oup.com
During the formation of a transcriptionally active macronucleus, ciliated protozoa excise large
numbers of interstitial segments of DNA (internal eliminated sequences; lESs) from their …

Genetic code deviations in the ciliates: evidence for multiple and independent events.

AB Tourancheau, N Tsao, LA Klobutcher… - The EMBO …, 1995 - embopress.org
In several species of ciliates, the universal stop codons UAA and UAG are translated into
glutamine, while in the euplotids, the glutamine codon usage is normal, but UGA appears to be …

Position-dependent termination and widespread obligatory frameshifting in Euplotes translation

…, F Xie, VA Petyuk, RD Smith, LA Klobutcher… - Nature structural & …, 2017 - nature.com
The ribosome can change its reading frame during translation in a process known as programmed
ribosomal frameshifting. These rare events are supported by complex mRNA signals. …

The Tetrahymena thermophila Phagosome Proteome

…, RE Pearlman, KWM Siu, LA Klobutcher - Eukaryotic …, 2006 - Am Soc Microbiol
In vertebrates, phagocytosis occurs mainly in specialized cells of the immune system and
serves as a primary defense against invading pathogens, but it also plays a role in clearing …