Formation of the three primary germ layers during gastrulation is an essential step in the
establishment of the vertebrate body plan and is associated with major transcriptional changes …

Translesion synthesis (TLS) polymerases are specialized DNA polymerases capable of
inserting nucleotides opposite DNA lesions that escape removal by dedicated DNA repair …

Analysis of differential gene expression is crucial for the study of cell fate and behavior
during embryonic development. However, automated methods for the sensitive detection and …

Transcription is often stochastic. This is seemingly incompatible with the importance of gene
expression during development. Here we show that during zebrafish embryogenesis, …

Recent advances in organoid and genome editing technologies are allowing for perturbation
experiments at an unprecedented scale. However, before doing such experiments it is …

Formation of the three primary germ layers during gastrulation is an essential step in the
establishment of the vertebrate body plan. Recent studies employing single cell RNA-…

Single molecule fluorescence in situ hybridization (smFISH) is a method to visualize single
mRNA molecules. When combined with cellular and nuclear segmentation, transcripts can be …

The use of gas-phase ion mobility separation in LC-MS based bottom-up proteomic experiments
is described. A data-independent acquisition approach was employed in combination …

… due to the larger amount of sample required for LC/MS analysis. In this work, we introduce
an online two dimensional capillary scale LC setup (0.3 mm ID) for comprehensive peptide …

Now that the sequencing of genomes has become routine, understanding how a given
genome is used in different ways to obtain cell type diversity in an organism is the next frontier. …