… might not lead to the same conclusions as in situ experiments in which specific enzymes
are inhibited in the presence of all regulators at physiological levels (see Krasinska et al., …

What do we know about Ki-67, apart from its usefulness as a cell proliferation biomarker in
histopathology? Discovered in 1983, the protein and its regulation of expression and …

10.7554/eLife.13722.001 Antigen Ki-67 is a nuclear protein expressed in proliferating
mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, …

… HDF at 25% confluency were incubated with medium supplemented with 2 mmol/L thymidine
or 2 mmol/L hydroxyurea for 24 hours. Cells were released from G 1 –S block by washing …

In eukaryotes, cyclin-dependent kinases (CDKs) are required for the onset of DNA replication
and mitosis, and distinct CDK–cyclin complexes are activated sequentially throughout the …

Adaptive therapy (AT) aims to control tumour burden by maintaining therapy-sensitive cells
to exploit their competition with resistant cells. This relies on the assumption that resistant …

Ki-67 is a nuclear protein that is expressed in all proliferating vertebrate cells. Here, we
demonstrate that, although Ki-67 is not required for cell proliferation, its genetic ablation inhibits …

Nuclear actin regulates transcriptional programmes in a manner dependent on its levels
and polymerisation state. This dynamics is determined by the balance of nucleocytoplasmic …

Since the mid 1970s, cancer has been described as a process of Darwinian evolution, with
somatic cellular selection and evolution being the fundamental processes leading to …

Cell cycle transitions result from global changes in protein phosphorylation states triggered
by cyclin-dependent kinases (CDKs). To understand how this complexity produces an …