We describe the complete synthesis, assembly, debugging, and characterization of a
synthetic 404,963 bp chromosome, synIX (synthetic chromosome IX). Combined chromosome …

Pioneering advances in genome engineering, and specifically in genome writing, have
revolutionized the field of synthetic biology, propelling us toward the creation of synthetic …

… Laura H McCulloch and Sudarshan Pinglay authors contributed equally to this work. … , was
used to inoculate 400–500 ml of LB kanamycin (25 μg/ml) cultures, which were grown for 24 h …

Whether synthetic genomes can power life has attracted broad interest in the synthetic biology
field. Here, we report de novo synthesis of the largest eukaryotic chromosome thus far, …

We designed and synthesized synI, which is ∼21.6% shorter than native chrI, the smallest
chromosome in Saccharomyces cerevisiae. SynI was designed for attachment to another …

Abstract Design and large-scale synthesis of DNA has been applied to the functional study
of viral and microbial genomes. New and expanded technology development is required to …

Overwriting counterselectable markers is an efficient strategy for removing wild-type DNA or
replacing it with payload DNA of interest. Currently, one bottleneck of efficient genome …

… McCulloch, MD, began lecturing to … McCulloch, MD gave a landmark paper entitled, ‘The
Veterinarian and Human Health-Care Systems: Issues and Boundaries’. Dr McCulloch also …

By combining genome-wide association data from 8,130 individuals with type 2 diabetes (T2D)
and 38,987 controls of European descent and following up previously unidentified meta-…

Levels of circulating glucose are tightly regulated. To identify new loci influencing glycemic
traits, we performed meta-analyses of 21 genome-wide association studies informative for …