10.7554/eLife.06074.001 Bruton's tyrosine kinase (Btk), a Tec-family tyrosine kinase, is
essential for B-cell function. We present crystallographic and biochemical analyses of Btk, which …

Tyrosine kinases were first discovered as the protein products of viral oncogenes. We now
know that this large family of metazoan enzymes includes nearly one hundred structurally …

Lipid miscibility phase separation has long been considered to be a central element of cell
membrane organization. More recently, protein condensation phase transitions, into three-…

The transformation of molecular binding events into cellular decisions is the basis of most
biological signal transduction. A fundamental challenge faced by these systems is that reliance …

Cancer mutations in Ras occur predominantly at three hotspots: Gly 12, Gly 13, and Gln 61.
Previously, we reported that deep mutagenesis of H-Ras using a bacterial assay identified …

We reconstitute a phosphotyrosine-mediated protein condensation phase transition of the ∼200
residue cytoplasmic tail of the epidermal growth factor receptor (EGFR) and the adaptor …

Holliday junctions play a central role in genetic recombination, DNA repair and other cellular
processes. We combine simulations and experiments to evaluate the ability of the 3SPN.2 …

The Tec-family kinase Btk contains a lipid-binding Pleckstrin homology and Tec homology (PH-TH)
module connected by a proline-rich linker to a ‘Src module’, an SH3-SH2-kinase unit …

Holliday Junctions are critical DNA intermediates central to double strand break repair and
homologous recombination. The junctions can adopt two general forms: open and stacked-X, …

We reconstitute a phosphotyrosine-mediated protein condensation phase transition of the ∼200
residue cytoplasmic tail of the epidermal growth factor receptor (EGFR) and the adaptor …