Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter

M Hohl, LM Hürlimann, S Böhm… - Proceedings of the …, 2014 - National Acad Sciences
ATP binding cassette (ABC) transporters mediate vital transport processes in every living
cell. ATP hydrolysis, which fuels transport, displays positive cooperativity in numerous ABC …

The ABC exporter IrtAB imports and reduces mycobacterial siderophores

…, P Egloff, I Zimmermann, CAJ Hutter, LM Hürlimann… - Nature, 2020 - nature.com
Intracellular replication of the deadly pathogen Mycobacterium tuberculosis relies on the
production of small organic molecules called siderophores that scavenge iron from host …

Sybodies targeting the SARS-CoV-2 receptor-binding domain

…, M Wyss, P Egloff, M Sorgenfrei, LM Hürlimann… - BioRxiv, 2020 - biorxiv.org
The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, has resulted in a
global health and economic crisis of unprecedented scale. The high transmissibility of SARS-…

[HTML][HTML] The extracellular gate shapes the energy profile of an ABC exporter

CAJ Hutter, MH Timachi, LM Hürlimann… - Nature …, 2019 - nature.com
ABC exporters harness the energy of ATP to pump substrates across membranes.
Extracellular gate opening and closure are key steps of the transport cycle, but the underlying …

Biparatopic sybodies neutralize SARS‐CoV‐2 variants of concern and mitigate drug resistance

…, JC Earp, P Egloff, M Sorgenfrei, LM Hürlimann… - EMBO …, 2022 - embopress.org
The ongoing COVID‐19 pandemic represents an unprecedented global health crisis. Here,
we report the identification of a synthetic nanobody (sybody) pair, Sb#15 and Sb#68, that …

The heterodimeric ABC transporter EfrCD mediates multidrug efflux in Enterococcus faecalis

LM Hürlimann, V Corradi, M Hohl… - Antimicrobial agents …, 2016 - Am Soc Microbiol
Nosocomial infections with Enterococcus faecalis are an emerging health problem. However,
drug efflux pumps contributing to intrinsic drug resistance are poorly studied in this Gram-…

Deep mutational scan of a drug efflux pump reveals its structure–function landscape

…, K Ehrenbolger, CAJ Hutter, LM Hürlimann… - Nature chemical …, 2023 - nature.com
Drug efflux is a common resistance mechanism found in bacteria and cancer cells, but studies
providing comprehensive functional insights are scarce. In this study, we performed deep …

Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG

…, M Dal Molin, FM Arnold, LM Hürlimann… - Molecular …, 2019 - Wiley Online Library
The major facilitator superfamily transporter Rv1410 and the lipoprotein LprG (Rv1411) are
encoded by a conserved two‐gene operon and contribute to virulence in Mycobacterium …

Split tasks of asymmetric nucleotide‐binding sites in the heterodimeric ABC exporter EfrCD

LM Hürlimann, M Hohl, MA Seeger - The FEBS journal, 2017 - Wiley Online Library
Many heterodimeric ATP‐binding cassette ( ABC ) exporters evolved asymmetric ATP ‐binding
sites containing a degenerate site incapable of ATP hydrolysis due to noncanonical …

Biomolecules capturing live bacteria from clinical samples

M Sorgenfrei, LM Hürlimann, MM Remy… - Trends in biochemical …, 2022 - cell.com
Rapid phenotypic antimicrobial susceptibility testing (AST) requires the enrichment of live
bacteria from patient samples, which is particularly challenging in the context of life-…