Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter
M Hohl, LM Hürlimann, S Böhm… - Proceedings of the …, 2014 - National Acad Sciences
ATP binding cassette (ABC) transporters mediate vital transport processes in every living
cell. ATP hydrolysis, which fuels transport, displays positive cooperativity in numerous ABC …
cell. ATP hydrolysis, which fuels transport, displays positive cooperativity in numerous ABC …
The ABC exporter IrtAB imports and reduces mycobacterial siderophores
…, P Egloff, I Zimmermann, CAJ Hutter, LM Hürlimann… - Nature, 2020 - nature.com
Intracellular replication of the deadly pathogen Mycobacterium tuberculosis relies on the
production of small organic molecules called siderophores that scavenge iron from host …
production of small organic molecules called siderophores that scavenge iron from host …
Sybodies targeting the SARS-CoV-2 receptor-binding domain
…, M Wyss, P Egloff, M Sorgenfrei, LM Hürlimann… - BioRxiv, 2020 - biorxiv.org
The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, has resulted in a
global health and economic crisis of unprecedented scale. The high transmissibility of SARS-…
global health and economic crisis of unprecedented scale. The high transmissibility of SARS-…
[HTML][HTML] The extracellular gate shapes the energy profile of an ABC exporter
CAJ Hutter, MH Timachi, LM Hürlimann… - Nature …, 2019 - nature.com
ABC exporters harness the energy of ATP to pump substrates across membranes.
Extracellular gate opening and closure are key steps of the transport cycle, but the underlying …
Extracellular gate opening and closure are key steps of the transport cycle, but the underlying …
Biparatopic sybodies neutralize SARS‐CoV‐2 variants of concern and mitigate drug resistance
…, JC Earp, P Egloff, M Sorgenfrei, LM Hürlimann… - EMBO …, 2022 - embopress.org
The ongoing COVID‐19 pandemic represents an unprecedented global health crisis. Here,
we report the identification of a synthetic nanobody (sybody) pair, Sb#15 and Sb#68, that …
we report the identification of a synthetic nanobody (sybody) pair, Sb#15 and Sb#68, that …
The heterodimeric ABC transporter EfrCD mediates multidrug efflux in Enterococcus faecalis
Nosocomial infections with Enterococcus faecalis are an emerging health problem. However,
drug efflux pumps contributing to intrinsic drug resistance are poorly studied in this Gram-…
drug efflux pumps contributing to intrinsic drug resistance are poorly studied in this Gram-…
Deep mutational scan of a drug efflux pump reveals its structure–function landscape
…, K Ehrenbolger, CAJ Hutter, LM Hürlimann… - Nature chemical …, 2023 - nature.com
Drug efflux is a common resistance mechanism found in bacteria and cancer cells, but studies
providing comprehensive functional insights are scarce. In this study, we performed deep …
providing comprehensive functional insights are scarce. In this study, we performed deep …
Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG
…, M Dal Molin, FM Arnold, LM Hürlimann… - Molecular …, 2019 - Wiley Online Library
The major facilitator superfamily transporter Rv1410 and the lipoprotein LprG (Rv1411) are
encoded by a conserved two‐gene operon and contribute to virulence in Mycobacterium …
encoded by a conserved two‐gene operon and contribute to virulence in Mycobacterium …
Split tasks of asymmetric nucleotide‐binding sites in the heterodimeric ABC exporter EfrCD
LM Hürlimann, M Hohl, MA Seeger - The FEBS journal, 2017 - Wiley Online Library
Many heterodimeric ATP‐binding cassette ( ABC ) exporters evolved asymmetric ATP ‐binding
sites containing a degenerate site incapable of ATP hydrolysis due to noncanonical …
sites containing a degenerate site incapable of ATP hydrolysis due to noncanonical …
Biomolecules capturing live bacteria from clinical samples
M Sorgenfrei, LM Hürlimann, MM Remy… - Trends in biochemical …, 2022 - cell.com
Rapid phenotypic antimicrobial susceptibility testing (AST) requires the enrichment of live
bacteria from patient samples, which is particularly challenging in the context of life-…
bacteria from patient samples, which is particularly challenging in the context of life-…