Probing the architecture, mechanism, and dynamics of genome folding is fundamental to
our understanding of genome function in homeostasis and disease. Most chromosome …

The RNA-guided CRISPR-associated protein Cas9 is used for genome editing, transcriptional
modulation, and live-cell imaging. Cas9-guide RNA complexes recognize and cleave …

Oncogene amplification on extrachromosomal DNA (ecDNA) is a common event, driving
aggressive tumor growth, drug resistance and shorter survival. Currently, the impact of …

Expanding the palette of fluorescent dyes is vital to push the frontier of biological imaging.
Although rhodamine dyes remain the premier type of small-molecule fluorophore owing to …

10.7554/eLife.22280.001 During mitosis, transcription is shut off, chromatin condenses, and
most transcription factors (TFs) are reported to be excluded from chromosomes. How do …

Fluorescence microscopy relies on dyes that absorb and then emit photons. In addition to
fluorescence, fluorophores can undergo photochemical processes that decrease quantum …

Although regulation of histone methylation is believed to contribute to embryonic stem cell (ESC)
self‐renewal, the mechanisms remain obscure. We show here that the histone H3 …

Extrachromosomal DNA (ecDNA) is prevalent in human cancers and mediates high expression
of oncogenes through gene amplification and altered gene regulation 1 . Gene induction …

10.7554/eLife.35621.001 Maintenance of transcription programs is challenged during mitosis
when chromatin becomes condensed and transcription is silenced. How do the daughter …

To image the accessible genome at nanometer scale in situ, we developed three-dimensional
assay for transposase-accessible chromatin-photoactivated localization microscopy (3D …