A genome-scale shRNA resource for transgenic RNAi in Drosophila
Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded
hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene …
hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene …
The transgenic RNAi project at Harvard Medical School: resources and validation
…, S Randkelv, C Kelley, P Namgyal, C Villalta, LP Liu… - Genetics, 2015 - academic.oup.com
To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi
Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: …
Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: …
Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9
The ability to engineer genomes in a specific, systematic, and cost-effective way is critical
for functional genomic studies. Recent advances using the CRISPR-associated single-guide …
for functional genomic studies. Recent advances using the CRISPR-associated single-guide …
A Drosophila resource of transgenic RNAi lines for neurogenetics
JQ Ni, LP Liu, R Binari, R Hardy, HS Shim… - Genetics, 2009 - academic.oup.com
Conditional expression of hairpin constructs in Drosophila is a powerful method to disrupt
the activity of single genes with a spatial and temporal resolution that is impossible, or …
the activity of single genes with a spatial and temporal resolution that is impossible, or …
Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster
JQ Ni, M Markstein, R Binari, B Pfeiffer, LP Liu… - Nature …, 2008 - nature.com
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged
in recent years as a method of choice in functional genomic studies. To date, upstream …
in recent years as a method of choice in functional genomic studies. To date, upstream …
[PDF][PDF] Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila
…, SJ Yang, HH Qiao, X Wang, Q Hu, P Deng, LP Liu… - Cell reports, 2014 - cell.com
The CRISPR/Cas9 system has recently emerged as a powerful tool for functional genomic
studies in Drosophila melanogaster. However, single-guide RNA (sgRNA) parameters …
studies in Drosophila melanogaster. However, single-guide RNA (sgRNA) parameters …
[HTML][HTML] Regulation of lipogenesis by cyclin-dependent kinase 8–mediated control of SREBP-1
…, XJ Xie, J Zhou, Y Sun, ES Yang, LP Liu… - The Journal of …, 2012 - Am Soc Clin Investig
Altered lipid metabolism underlies several major human diseases, including obesity and type
2 diabetes. However, lipid metabolism pathophysiology remains poorly understood at the …
2 diabetes. However, lipid metabolism pathophysiology remains poorly understood at the …
An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination
Previously, we described a large collection of Drosophila strains that each carry an artificial
exon containing a T2AGAL4 cassette inserted in an intron of a target gene based on CRISPR…
exon containing a T2AGAL4 cassette inserted in an intron of a target gene based on CRISPR…
Drosophila ribosomal proteins are associated with linker histone H1 and suppress gene transcription
JQ Ni, LP Liu, D Hess, J Rietdorf… - Genes & …, 2006 - genesdev.cshlp.org
The dynamics and function of ribosomal proteins in the cell nucleus remain enigmatic. Here
we provide evidence that specific components of Drosophila melanogaster ribosomes …
we provide evidence that specific components of Drosophila melanogaster ribosomes …
[HTML][HTML] Piwi Is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary
…, S Wang, T Do, X Song, M Inaba, Y Nishimoto, L Liu… - PloS one, 2014 - journals.plos.org
The piRNA pathway plays an important role in maintaining genome stability in the germ line
by silencing transposable elements (TEs) from fly to mammals. As a highly conserved …
by silencing transposable elements (TEs) from fly to mammals. As a highly conserved …