The neocortex contains a multitude of cell types that are segregated into layers and functionally
distinct areas. To investigate the diversity of cell types across the mouse neocortex, here …

The primary motor cortex (M1) is essential for voluntary fine-motor control and is functionally
conserved across mammals 1 . Here, using high-throughput transcriptomic and epigenomic …

Elucidating the cellular architecture of the human cerebral cortex is central to understanding
our cognitive abilities and susceptibility to disease. Here we used single-nucleus RNA-…

To facilitate scalable profiling of single cells, we developed split-pool ligation-based transcriptome
sequencing (SPLiT-seq), a single-cell RNA-seq (scRNA-seq) method that labels the …

The isocortex and hippocampal formation (HPF) in the mammalian brain play critical roles
in perception, cognition, emotion, and learning. We profiled ∼1.3 million cells covering the …

Modern genetic approaches are powerful in providing access to diverse cell types in the brain
and facilitating the study of their function. Here, we report a large set of driver and reporter …

Transcriptomic profiling of complex tissues by single-nucleus RNA-sequencing (snRNA-seq)
affords some advantages over single-cell RNA-sequencing (scRNA-seq). snRNA-seq …

Single-cell measurements of cellular characteristics have been instrumental in understanding
the heterogeneous pathways that drive differentiation, cellular responses to signals, and …

Activity in the motor cortex predicts movements, seconds before they are initiated. This
preparatory activity has been observed across cortical layers, including in descending pyramidal …

The ventrolateral subdivision of the ventromedial hypothalamus (VMHvl) contains ∼4,000
neurons that project to multiple targets and control innate social behaviors including …