Duplication and segregation of chromosomes involves dynamic reorganization of their
internal structure by conserved architectural proteins, including the structural maintenance of …

The Sc2.0 project is building a eukaryotic synthetic genome from scratch. A major milestone
has been achieved with all individual Sc2.0 chromosomes assembled. Here, we describe …

Cohesin, a member of the SMC complex family, holds sister chromatids together but also
shapes chromosomes by promoting the formation of long-range intra-chromatid loops, a …

In chromosome conformation capture experiments (Hi‐C), the accuracy with which contacts
are detected varies due to the uneven distribution of restriction sites along genomes. In …

Pioneering advances in genome engineering, and specifically in genome writing, have
revolutionized the field of synthetic biology, propelling us toward the creation of synthetic …

Cohesin is a regulator of genome architecture with roles in sister chromatid cohesion and
chromosome compaction. The recruitment and mobility of cohesin complexes on DNA is …

Forcing budding yeast to chromatinize their DNA with human histones manifests an abrupt
fitness cost. We previously proposed chromosomal aneuploidy and missense mutations as …

Whether synthetic genomes can power life has attracted broad interest in the synthetic biology
field. Here, we report de novo synthesis of the largest eukaryotic chromosome thus far, …

Physical contacts between distant loci contribute to regulate genome function. However, the
molecular mechanisms responsible for settling and maintaining such interactions remain …

The nucleolus is the most prominent membraneless compartment within the nucleus—dedicated
to the metabolism of ribosomal RNA. Nucleoli are composed of hundreds of ribosomal …