Role of melanin as a scavenger of active oxygen species

J Bustamante, L Bredeston, G Malanga… - Pigment cell …, 1993 - Wiley Online Library
The protective role of melanin, either synthetic or derived from a metastatic lung melanoma
nodule, was studied in terms of its ability to interact with active oxygen species (O 2 , H 2 O 2 , …

[HTML][HTML] Golgi and endoplasmic reticulum functions take place in different subcellular compartments of Entamoeba histolytica

LM Bredeston, CE Caffaro, J Samuelson… - Journal of Biological …, 2005 - ASBMB
Entamoeba histolytica is a protozoan parasite that causes dysentery in developing countries
of Africa, Asia, and Latin America. The lack of a defined Golgi apparatus in E. histolytica as …

Deglycosylated RBD produced in Pichia pastoris as a low-cost sera COVID-19 diagnosis tool and a vaccine candidate

T Idrovo-Hidalgo, MF Pignataro, LM Bredeston… - …, 2024 - academic.oup.com
During the COVID-19 outbreak, numerous tools including protein-based vaccines have been
developed. The methylotrophic yeast Pichia pastoris (synonymous to Komagataella phaffii) …

[HTML][HTML] Activation of Archaeoglobus fulgidus Cu+-ATPase CopA by cysteine

Y Yang, AK Mandal, LM Bredeston… - … et Biophysica Acta (BBA …, 2007 - Elsevier
CopA, a thermophilic ATPase from Archaeoglobus fulgidus, drives the outward movement
of Cu + across the cell membrane. Millimolar concentration of Cys dramatically increases (≅…

[HTML][HTML] Loss of autoinhibition of the plasma membrane Ca2+ pump by substitution of aspartic 170 by asparagine: activation of plasma membrane calcium ATPase 4 …

LM Bredeston, HP Adamo - Journal of Biological Chemistry, 2004 - ASBMB
The plasma membrane calcium ATPase (PMCA) actively transports Ca 2+ from the cytosol
to the extra cellular space. The C-terminal segment of the PMCA functions as an inhibitory …

Pre-steady-state phosphorylation and dephosphorylation of detergent-purified plasma-membrane Ca2+-ATPase

LM Bredeston, AF Rega - Biochemical Journal, 2002 - portlandpress.com
Pre-steady-state phosphorylation and dephosphorylation of purified and phospholipid-depleted
plasma-membrane Ca 2+ -ATPase (PMCA) solubilized in the detergent polyoxyethylene …

[HTML][HTML] Conserved Glu-47 and Lys-50 residues are critical for UDP-N-acetylglucosamine/UMP antiport activity of the mouse Golgi-associated transporter Slc35a3

…, B Ebert, C Rautengarten, LM Bredeston - Journal of Biological …, 2019 - ASBMB
Nucleotide sugar transporters (NSTs) regulate the flux of activated sugars from the cytosol
into the lumen of the Golgi apparatus where glycosyltransferases use them for the …

[HTML][HTML] The N-terminal region of the plasma membrane Ca2+ pump does not separate from the main catalytic fragments after proteolysis

HP Adamo, ME Grimaldi, LM Bredeston - Biochimica et Biophysica Acta …, 2000 - Elsevier
The purified plasma membrane Ca 2+ pump (PMCA) was digested with trypsin, and the
proteolytic products were identified by immunoblotting with monoclonal antibodies JA9 or 5F10 …

[HTML][HTML] The promiscuous phosphomonoestearase activity of Archaeoglobus fulgidus CopA, a thermophilic Cu+ transport ATPase

LM Bredeston, FLG Flecha - Biochimica et Biophysica Acta (BBA) …, 2016 - Elsevier
Membrane transport P-type ATPases display two characteristic enzymatic activities: a principal
ATPase activity provides the driving force for ion transport across biological membranes, …

[HTML][HTML] Phosphatidylcholine makes specific activity of the purified Ca2+-ATPase from plasma membranes independent of enzyme concentration

LM Bredeston, AF Rega - Biochimica et Biophysica Acta (BBA) …, 1999 - Elsevier
Ca 2+ -ATPase of plasma membranes (PMCA) was isolated from either human or pig red
cells by calmodulin-affinity chromatography and supplemented with phosphatidylcholine (PC). …