User profiles for Luis F. Schachner
Luis F. SchachnerGenentech, Inc. Verified email at gene.com Cited by 1042 |
[HTML][HTML] Best practices and benchmarks for intact protein analysis for top-down mass spectrometry
…, CM Rawlins, CJ DeHart, L Fornelli, LF Schachner… - Nature …, 2019 - nature.com
One gene can give rise to many functionally distinct proteoforms, each of which has a
characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins …
characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins …
Top-down characterization of endogenous protein complexes with native proteomics
Protein complexes exhibit great diversity in protein membership, post-translational modifications
and noncovalent cofactors, enabling them to function as the actuators of many important …
and noncovalent cofactors, enabling them to function as the actuators of many important …
The biosynthesis of methanobactin
…, RJ Martinie, P Gao, CJ DeHart, LF Schachner… - Science, 2018 - science.org
Metal homeostasis poses a major challenge to microbes, which must acquire scarce
elements for core metabolic processes. Methanobactin, an extensively modified copper-chelating …
elements for core metabolic processes. Methanobactin, an extensively modified copper-chelating …
[HTML][HTML] Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
SY Ro, LF Schachner, CW Koo, R Purohit… - Nature …, 2019 - nature.com
Aerobic methane oxidation is catalyzed by particulate methane monooxygenase (pMMO), a
copper-dependent, membrane metalloenzyme composed of subunits PmoA, PmoB, and …
copper-dependent, membrane metalloenzyme composed of subunits PmoA, PmoB, and …
[HTML][HTML] Top-down proteomics: Where we are, where we are going?
After some years spent increasing metrics of proteome coverage, the field of proteomics has
increasingly focused on the quality of information generated during interrogation of living …
increasingly focused on the quality of information generated during interrogation of living …
Individual ion mass spectrometry enhances the sensitivity and sequence coverage of top-down mass spectrometry
…, RD Melani, BJ Des Soye, LF Schachner… - Journal of proteome …, 2020 - ACS Publications
Charge detection mass spectrometry (CDMS) is mainly utilized to determine the mass of intact
molecules. Previous applications of CDMS have determined the mass-to-charge ratio and …
molecules. Previous applications of CDMS have determined the mass-to-charge ratio and …
Decoding the protein composition of whole nucleosomes with Nuc-MS
Current proteomic approaches disassemble and digest nucleosome particles, blurring readouts
of the ‘histone code’. To preserve nucleosome-level information, we developed Nuc-MS, …
of the ‘histone code’. To preserve nucleosome-level information, we developed Nuc-MS, …
Standard proteoforms and their complexes for native mass spectrometry
LF Schachner, AN Ives, JP McGee… - Journal of The …, 2019 - ACS Publications
Native mass spectrometry (nMS) is a technique growing at the interface of analytical chemistry,
structural biology, and proteomics that enables the detection and partial characterization …
structural biology, and proteomics that enables the detection and partial characterization …
Native vs denatured: an in depth investigation of charge state and isotope distributions
New tools and techniques have dramatically accelerated the field of structural biology over
the past several decades. One potent and relatively new technique that is now being utilized …
the past several decades. One potent and relatively new technique that is now being utilized …
Revving an engine of human metabolism: activity enhancement of triosephosphate isomerase via hemi-phosphorylation
LF Schachner, BD Soye, S Ro, GE Kenney… - ACS chemical …, 2022 - ACS Publications
Triosephosphate isomerase (TPI) performs the 5th step in glycolysis, operates near the limit
of diffusion, and is involved in “moonlighting” functions. Its dimer was found singly …
of diffusion, and is involved in “moonlighting” functions. Its dimer was found singly …