User profiles for Luis F. Schachner

Luis F. Schachner

Genentech, Inc.
Verified email at gene.com
Cited by 1042

[HTML][HTML] Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

…, CM Rawlins, CJ DeHart, L Fornelli, LF Schachner… - Nature …, 2019 - nature.com
One gene can give rise to many functionally distinct proteoforms, each of which has a
characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins …

Top-down characterization of endogenous protein complexes with native proteomics

…, HS Seckler, PF Doubleday, LF Schachner… - Nature chemical …, 2018 - nature.com
Protein complexes exhibit great diversity in protein membership, post-translational modifications
and noncovalent cofactors, enabling them to function as the actuators of many important …

The biosynthesis of methanobactin

…, RJ Martinie, P Gao, CJ DeHart, LF Schachner… - Science, 2018 - science.org
Metal homeostasis poses a major challenge to microbes, which must acquire scarce
elements for core metabolic processes. Methanobactin, an extensively modified copper-chelating …

[HTML][HTML] Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase

SY Ro, LF Schachner, CW Koo, R Purohit… - Nature …, 2019 - nature.com
Aerobic methane oxidation is catalyzed by particulate methane monooxygenase (pMMO), a
copper-dependent, membrane metalloenzyme composed of subunits PmoA, PmoB, and …

[HTML][HTML] Top-down proteomics: Where we are, where we are going?

L Fornelli, TK Toby, LF Schachner… - Journal of …, 2018 - ncbi.nlm.nih.gov
After some years spent increasing metrics of proteome coverage, the field of proteomics has
increasingly focused on the quality of information generated during interrogation of living …

Individual ion mass spectrometry enhances the sensitivity and sequence coverage of top-down mass spectrometry

…, RD Melani, BJ Des Soye, LF Schachner… - Journal of proteome …, 2020 - ACS Publications
Charge detection mass spectrometry (CDMS) is mainly utilized to determine the mass of intact
molecules. Previous applications of CDMS have determined the mass-to-charge ratio and …

Decoding the protein composition of whole nucleosomes with Nuc-MS

LF Schachner, K Jooß, MA Morgan, A Piunti… - Nature …, 2021 - nature.com
Current proteomic approaches disassemble and digest nucleosome particles, blurring readouts
of the ‘histone code’. To preserve nucleosome-level information, we developed Nuc-MS, …

Standard proteoforms and their complexes for native mass spectrometry

LF Schachner, AN Ives, JP McGee… - Journal of The …, 2019 - ACS Publications
Native mass spectrometry (nMS) is a technique growing at the interface of analytical chemistry,
structural biology, and proteomics that enables the detection and partial characterization …

Native vs denatured: an in depth investigation of charge state and isotope distributions

JO Kafader, RD Melani, LF Schachner… - Journal of the …, 2020 - ACS Publications
New tools and techniques have dramatically accelerated the field of structural biology over
the past several decades. One potent and relatively new technique that is now being utilized …

Revving an engine of human metabolism: activity enhancement of triosephosphate isomerase via hemi-phosphorylation

LF Schachner, BD Soye, S Ro, GE Kenney… - ACS chemical …, 2022 - ACS Publications
Triosephosphate isomerase (TPI) performs the 5th step in glycolysis, operates near the limit
of diffusion, and is involved in “moonlighting” functions. Its dimer was found singly …