User profiles for Lukas M Weber
 | Lukas M. WeberAssistant Professor, Department of Biostatistics, Boston University Verified email at bu.edu Cited by 3198 |
High-dimensional single-cell analysis predicts response to anti-PD-1 immunotherapy
Immune-checkpoint blockade has revolutionized cancer therapy. In particular, inhibition of
programmed cell death protein 1 (PD-1) has been found to be effective for the treatment of …
programmed cell death protein 1 (PD-1) has been found to be effective for the treatment of …
Transcriptome-scale spatial gene expression in the human dorsolateral prefrontal cortex
We used the 10x Genomics Visium platform to define the spatial topography of gene expression
in the six-layered human dorsolateral prefrontal cortex. We identified extensive layer-…
in the six-layered human dorsolateral prefrontal cortex. We identified extensive layer-…
[HTML][HTML] CyTOF workflow: differential discovery in high-throughput high-dimensional cytometry datasets
High-dimensional mass and flow cytometry (HDCyto) experiments have become a method
of choice for high-throughput interrogation and characterization of cell populations. Here, we …
of choice for high-throughput interrogation and characterization of cell populations. Here, we …
Comparison of clustering methods for high‐dimensional single‐cell flow and mass cytometry data
LM Weber, MD Robinson - Cytometry Part A, 2016 - Wiley Online Library
Recent technological developments in high‐dimensional flow cytometry and mass cytometry
(CyTOF) have made it possible to detect expression levels of dozens of protein markers in …
(CyTOF) have made it possible to detect expression levels of dozens of protein markers in …
Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes
CRISPR-Cas9 enables efficient sequence-specific mutagenesis for creating somatic or germline
mutants of model organisms. Key constraints in vivo remain the expression and delivery …
mutants of model organisms. Key constraints in vivo remain the expression and delivery …
[HTML][HTML] diffcyt: Differential discovery in high-dimensional cytometry via high-resolution clustering
High-dimensional flow and mass cytometry allow cell types and states to be characterized
in great detail by measuring expression levels of more than 40 targeted protein markers per …
in great detail by measuring expression levels of more than 40 targeted protein markers per …
[HTML][HTML] Essential guidelines for computational method benchmarking
In computational biology and other sciences, researchers are frequently faced with a choice
between several computational methods for performing data analyses. Benchmarking …
between several computational methods for performing data analyses. Benchmarking …
[HTML][HTML] Statistical methods for detecting differentially methylated loci and regions
DNA methylation, the reversible addition of methyl groups at CpG dinucleotides, represents
an important regulatory layer associated with gene expression. Changed methylation status …
an important regulatory layer associated with gene expression. Changed methylation status …
An updated guide for the perplexed: cytometry in the high-dimensional era
High-dimensional cytometry experiments measuring 20–50 cellular markers have become
routine in many laboratories. The increased complexity of these datasets requires added rigor …
routine in many laboratories. The increased complexity of these datasets requires added rigor …
High-dimensional single-cell analysis reveals the immune signature of narcolepsy
Narcolepsy type 1 is a devastating neurological sleep disorder resulting from the destruction
of orexin-producing neurons in the central nervous system (CNS). Despite its striking …
of orexin-producing neurons in the central nervous system (CNS). Despite its striking …