Several macromolecular machines collaborate to produce eukaryotic messenger RNA. RNA
polymerase II (Pol II) translocates along genes that are up to millions of base pairs in length …

Protein-coding genes in eukaryotes are transcribed by RNA polymerase II (Pol II) and introns
are removed from pre-mRNA by the spliceosome. Understanding the time lag between Pol …

Coexpression of proteins in response to pathway-inducing signals is the founding paradigm
of gene regulation. However, it remains unexplored whether the relative abundance of co-…

Splicing is the removal of intron sequences from pre-mRNA by the spliceosome. Researchers
working in multiple model organisms–notably yeast, insects and mammalian cells–have …

Pre-mRNA splicing is accomplished by the spliceosome, a megadalton complex that
assembles de novo on each intron. Because spliceosome assembly and catalysis occur …

An important step toward understanding gene regulation is the elucidation of the time
necessary for the completion of individual steps. Measurement of reaction rates can reveal …

During the maternal-to-zygotic transition (MZT), transcriptionally silent embryos rely on post-transcriptional
regulation of maternal mRNAs until zygotic genome activation (ZGA). RNA-…

Modified nucleotides in non-coding RNAs, such as tRNAs and snRNAs, represent an important
layer of gene expression regulation through their ability to fine-tune mRNA maturation …

Nascent RNA sequencing has revealed that pre-mRNA splicing can occur shortly after
introns emerge from RNA polymerase II (RNA Pol II). Differences in co-transcriptional splicing …

Many steps of RNA processing occur during transcription by RNA polymerases. Co-transcriptional
activities are deemed commonplace in prokaryotes, in which the lack of membrane …