[HTML][HTML] Honey bees as biomonitors of environmental contaminants, pathogens, and climate change

…, JS Griffiths, GJ Bilodeau, M Rott, MM Guarna - Ecological …, 2022 - Elsevier
Monitoring the environment for pollution, pesticides, and pathogens is crucial for protecting
human, agriculture, and overall ecosystem health. Diverse strategies ranging from physical …

An anti-infective peptide that selectively modulates the innate immune response

…, P Hamill, JJ Yu, Y Li, O Donini, MM Guarna… - Nature …, 2007 - nature.com
We show that an innate defense–regulator peptide (IDR-1) was protective in mouse models
of infection with important Gram-positive and Gram-negative pathogens, including methicillin…

Vulnerability of honey bee queens to heat-induced loss of fertility

…, R Underwood, J Milone, LJ Foster, MM Guarna… - Nature …, 2020 - nature.com
All species need to reproduce to maintain viable populations, but heat stress kills sperm cells
across the animal kingdom and rising frequencies of heat waves are a threat to biodiversity…

[HTML][HTML] Correlation of proteome-wide changes with social immunity behaviors provides insight into resistance to the parasitic mite, Varroa destructor, in the honey bee …

R Parker, MM Guarna, AP Melathopoulos, KM Moon… - Genome biology, 2012 - Springer
… with stability maintained in PBS (50 mM K 2 HPO 4 , 150 mM NaCl, pH 7.4) containing … mM
Tris-HCl, 150 mM NaCl, 1% NP-40, 20 mM dithiolthretitol in a Fast Prep bead mill with 2.8 mm

[HTML][HTML] Sequestosome-1/p62 is the key intracellular target of innate defense regulator peptide

…, Y Li, L Thorson, REW Hancock, MM Guarna… - Journal of biological …, 2009 - ASBMB
Innate defense regulator-1 (IDR-1) is a synthetic peptide with no antimicrobial activity that
enhances microbial infection control while suppressing inflammation. Previously, the effects of …

On‐line monitoring and control of methanol concentration in shake‐flask cultures of Pichia pastoris

MM Guarna, GJ Lesnicki, BM Tam… - Biotechnology and …, 1997 - Wiley Online Library
The methylotrophic yeast Pichia pastoris can be used to express recombinant genes at high
levels under the control of the methanol‐inducible alcohol oxidase 1 (AOX1) promoter. …

[HTML][HTML] Ecological Adaptation of Diverse Honey Bee (Apis mellifera) Populations

…, AP Melathopoulos, R White, SF Pernal, MM Guarna… - PLoS …, 2010 - journals.plos.org
… in 500 mM NaCH 3 COO (pH 8.0) and derivatized by addition of 20 µl of 200 mM CH 2 O (light)
or 200 mM C 2 H 2 O (medium) and 6 µL of 1 M NaBH3CN to both or 200 mM 13 C 2 H 2 …

Alternative reading frame selection mediated by a tRNA-like domain of an internal ribosome entry site

…, MMY Chan, JW Gouw, MM Guarna… - Proceedings of the …, 2012 - National Acad Sciences
… For optimal IRES activity, a final concentration of 0.5 mM MgCl 2 /40 mM KOAc was used.
Proteins were analyzed by 16% SDS-PAGE and by using a Typhoon imager (Amersham). …

[HTML][HTML] Peptide biomarkers used for the selective breeding of a complex polygenic trait in honey bees

MM Guarna, SE Hoover, E Huxter, H Higo, KM Moon… - Scientific reports, 2017 - nature.com
… of 5 mM tris(2-carboxyethyl) phosphine in 50 mM ammonium … of 20 mM iodoacetamide (in
50 mM ammonium bicarbonate) … µL of 20 mM dithiothreitol (in 50 mM ammonium bicarbonate) …

[HTML][HTML] Area Wide Monitoring of Plant and Honey Bee (Apis mellifera) Viruses in Blueberry (Vaccinium corymbosum) Agroecosystems Facilitated by Honey Bee …

…, A Smith, A Wang, GJ Bilodeau, SF Pernal, MM Guarna… - Viruses, 2023 - mdpi.com
Healthy agroecosystems are dependent on a complex web of factors and inter-species
interactions. Flowers are hubs for pathogen transmission, including the horizontal or vertical …