Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi

…, JS Hawkins, CHS Lu, MR Silvis, MM Harden… - Nature …, 2019 - nature.com
… Exponential-phase cultures were then back diluted to an A 600 nm of 0.02 and grown until
A 600 nm 0.2 before inducing rapI expression with 1 mM IPTG for 1 h. Donor and recipient …

Acid-adapted strains of Escherichia coli K-12 obtained by experimental evolution

MM Harden, A He, K Creamer, MW Clark… - Applied and …, 2015 - Am Soc Microbiol
… using the growth media LBK medium with 100 mM PIPES [piperazine-N,N′-bis(2-ethanesulfonic
acid)], pH 6.5, and 15 mM benzoic acid and LBK medium with 150 mM TAPS (3-{[1,3-…

A respiratory chain controlled signal transduction cascade in the mitochondrial intermembrane space mediates hydrogen peroxide signaling

…, CX Huang, MM Harden… - Proceedings of the …, 2015 - National Acad Sciences
… Stimulation with a minimum of 1 mM H 2 O 2 for 5 min or 5 mM for 10 min was required in
primary B cells and MEFs, respectively, to detect robust H 2 O 2 -induced tyrosine …

[HTML][HTML] Interactions between mobile genetic elements: An anti-phage gene in an integrative and conjugative element protects host cells from predation by a …

CM Johnson, MM Harden, AD Grossman - PLoS Genetics, 2022 - journals.plos.org
… (SMS agar contains 15 mM ammonium sulfate, 80 mM dibasic potassium phosphate, 44 mM
monobasic potassium phosphate, 3.4 mM trisodium citrate, 0.8 mM magnesium sulfate, and …

An integrative and conjugative element encodes an abortive infection system to protect host cells from predation by a bacteriophage

CM Johnson, MM Harden, AD Grossman - Biorxiv, 2020 - biorxiv.org
… (SMS agar contains 15 mM ammonium sulfate, 80 mM dibasic potassium phosphate, 44 mM
monobasic potassium phosphate, 3.4 mM trisodium citrate, 0.8 mM magnesium sulfate, and …

Toward a comprehensive catalog of global historical seismicity

…, ER Engdahl, DW Frazier, MM Harden… - Eos, Transactions …, 1997 - Wiley Online Library
The United States Geological Survey (USGS) and the Colorado School of Mines (CSM) have
initiated a project to locate more accurately all earthquakes recorded by instruments during …

A randomized trial of nurse‐administered behavioral interventions to manage anticipatory nausea and vomiting in chemotherapy

…, MJ Fisch, RL Bassett, MM Harden‐Harrison… - Cancer …, 2020 - Wiley Online Library
Purpose Chemotherapy side effects diminish quality of life and can lead to treatment delay.
Nausea and vomiting can occur prior to chemotherapy because of classical conditioning. We …

[HTML][HTML] Escherichia coli K-12 Survives Anaerobic Exposure at pH 2 without RpoS, Gad, or Hydrogenases, but Shows Sensitivity to Autoclaved Broth Products

DP Riggins, MJ Narvaez, KA Martinez, MM Harden… - PLoS …, 2013 - journals.plos.org
… Cultures were grown overnight in LBK buffered with 100 mM 2-(N-morpholino)ethanesulfonic
acid (MES) at pH 5.5 to up-regulate acid response systems [1]. Cultures were exposed to …

A review of the use of high power diode lasers in surface hardening

E Kennedy, G Byrne, DN Collins - Journal of Materials Processing …, 2004 - Elsevier
… wide and 0.65 mm deep hardened track with a hardness of 750 HV 0.05 was obtained. At a
… 22 mm and a processing speed of 200 mm/min, a 19 mm wide and 0.7 mm deep hardened

Microstructural changes during annealing of work-hardened mechanically milled metallic powders (overview)

Y Kimura, S Takaki - Materials Transactions, JIM, 1995 - jstage.jst.go.jp
hardening subjected by the prior MM treat— ment. In the case that powders are not work-hardened
However, when powders are extremely work-hardened to the level where crystalline …