User profiles for M. M. Mhlanga
musa m. mhlangaProfessor, Radboud Institute for Molecular Life Sciences, Verified email at mhlangalab.org Cited by 9167 |
[HTML][HTML] Defining trained immunity and its role in health and disease
…, JWM van der Meer, MM Mhlanga… - Nature Reviews …, 2020 - nature.com
Immune memory is a defining feature of the acquired immune system, but activation of the
innate immune system can also result in enhanced responsiveness to subsequent triggers. …
innate immune system can also result in enhanced responsiveness to subsequent triggers. …
[PDF][PDF] The intersection of epigenetics and metabolism in trained immunity
The last few years have witnessed an increasing body of evidence that challenges the
traditional view that immunological memory is an exclusive trait of the adaptive immune system. …
traditional view that immunological memory is an exclusive trait of the adaptive immune system. …
PALM and STORM: unlocking live‐cell super‐resolution
…, C Griffiths, E Hesper Rego, MM Mhlanga - …, 2011 - Wiley Online Library
Live‐cell fluorescence light microscopy has emerged as an important tool in the study of
cellular biology. The development of fluorescent markers in parallel with super‐resolution …
cellular biology. The development of fluorescent markers in parallel with super‐resolution …
QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ
To the Editor: Although conventional microscopes have a resolution limited by diffraction to
about half the wavelength of light, several recent advances have led to microscopy methods …
about half the wavelength of light, several recent advances have led to microscopy methods …
Visualizing the distribution and transport of mRNAs in living cells
DP Bratu, BJ Cha, MM Mhlanga… - Proceedings of the …, 2003 - National Acad Sciences
… -μl solution containing 40 mM Tris·HCl (pH 8.0), 10 mM MgSO 4 , 1 mM CaCl 2 , and 160 …
of a 125-μl solution containing 20 mM Tris·HCl (pH 8.0), 1 mM MgCl 2 , and 80 nM molecular …
of a 125-μl solution containing 20 mM Tris·HCl (pH 8.0), 1 mM MgCl 2 , and 80 nM molecular …
Spectral genotyping of human alleles
Accelerated by the human genome project [HN1], an increasing number of genetic variations,
many as small as a single nucleotide substitution [HN2], have been found to play a …
many as small as a single nucleotide substitution [HN2], have been found to play a …
Statin Therapy Reduces the Mycobacterium tuberculosis Burden in Human Macrophages and in Mice by Enhancing Autophagy and Phagosome Maturation
…, DM Lang, R Hurdayal, MM Mhlanga… - The Journal of …, 2014 - academic.oup.com
Background. Statins are cholesterol-lowering drugs, targeting HMG-CoA reductase, thereby
reducing the risk of coronary disorders and hypercholesterolemia. However, they also can …
reducing the risk of coronary disorders and hypercholesterolemia. However, they also can …
[PDF][PDF] BCG vaccination induces long-term functional reprogramming of human neutrophils
… In order to remove erythrocytes, the cells were treated twice with hypotonic lysis buffer (155
mM NH4Cl, 10 mM KHCO3) during incubation on ice for 15 minutes. Next, PMNs were …
mM NH4Cl, 10 mM KHCO3) during incubation on ice for 15 minutes. Next, PMNs were …
Using molecular beacons to detect single-nucleotide polymorphisms with real-time PCR
MM Mhlanga, L Malmberg - Methods, 2001 - Elsevier
… taining 200 nM of molecular beacon, 3.5 mM MgCl2, and 10 mM Tris–HCl, pH 8.0, in a
total volume of 50 μl should be prepared. Six tubes are used if doing a DNA Samples duplex …
total volume of 50 μl should be prepared. Six tubes are used if doing a DNA Samples duplex …
Immune genes are primed for robust transcription by proximal long noncoding RNAs located in nuclear compartments
… de-stained using 50 mM NH 4 HCO 3 /50% MeOH followed by in-gel protein reduction (50
mM DTT in 25 mM NH 4 HCO 3 ) and alkylation (55 mM iodoacetamide in 25 mM NH 4 HCO 3 …
mM DTT in 25 mM NH 4 HCO 3 ) and alkylation (55 mM iodoacetamide in 25 mM NH 4 HCO 3 …