[HTML][HTML] Functional identification of the hypoxanthine/guanine transporters YjcD and YgfQ and the adenine transporters PurP and YicO of Escherichia coli K-12

K Papakostas, M Botou, S Frillingos - Journal of Biological Chemistry, 2013 - ASBMB
… Fully grown cultures were diluted 10-fold, allowed to grow to midlogarithmic phase,
induced with isopropyl 1-thio-β-d-galactopyranoside (0.5 mm) for an additional 1.5 h at 37 C, …

Insight on specificity of uracil permeases of the NAT/NCS2 family from analysis of the transporter encoded in the pyrimidine utilization operon of Escherichia coli

M Botou, P Lazou, K Papakostas… - Molecular …, 2018 - Wiley Online Library
… different K M and V max /K M values with the two substrates. The K M values measured for
… /A308G display threefold lower K M with uracil and twofold higher K M with thymine. Of the …

[HTML][HTML] Cysteine-scanning analysis of helices TM8, TM9a, and TM9b and intervening loops in the YgfO xanthine permease: a carboxyl group is essential at ASP-276

…, E Georgopoulou, A Kallis, M Botou… - Journal of biological …, 2010 - ASBMB
… For kinetic analysis, putative inhibitors were used in the concentration range of 1 μm to 2
mm, and data were fitted to the equation y = B + (T − B)/(1 + 10 ((log IC50 − log x) h) ) for …

[HTML][HTML] Heterologous expression of the mammalian sodium-nucleobase transporter rSNBT1 in Leishmania tarentolae

A Doukas, E Karena, M Botou, K Papakostas… - … et Biophysica Acta (BBA …, 2019 - Elsevier
… Although not shown, the transport rate increased almost linearly with increasing concentrations
of [ 3 H]-uracil from 20 nΜ to 1 μΜ, implying that the K M for this substrate should be …

NAT/NCS2-hound: A webserver for the detection and evolutionary classification of prokaryotic and eukaryotic nucleobase-cation symporters of the NAT/NCS2 family

A Chaliotis, P Vlastaridis, C Ntountoumi, M Botou… - …, 2018 - academic.oup.com
Nucleobase transporters are important for supplying the cell with purines and/or pyrimidines,
for controlling the intracellular pool of nucleotides, and for obtaining exogenous nitrogen/…

Specificity profile of NAT/NCS2 purine transporters in Sinorhizobium (Ensifer) meliloti

M Botou, V Yalelis, P Lazou, I Zantza… - Molecular …, 2020 - Wiley Online Library
… uric acid with lower affinity (K M = 27.3 μΜ); SmLL8 and SmX28 are uric acid transporters
(K M = 3.5 and 21.4 μΜ, respectively); SmVC3 transports with high-affinity adenine (K M

Molecular and physiological characterization of the monosaccharide transporters gene family in Medicago truncatula

…, K Kalliampakou, M Botou, M Nikolaidis… - Journal of …, 2020 - academic.oup.com
… profiles of M. truncatula MST-encoding genes were in silico identified and retrieved from M.
… The MS source was held at 250 C and the quadrupole at 150 C and scanned from m/z 50 to …

[PDF][PDF] BBA-Biomembranes

A Doukas, E Karena, M Botou, K Papakostas… - jenabioscience.com
Recombinant expression systems for mammalian membrane transport proteins are often
limited by insufficient yields to support structural studies, inadequate post-translational …

Anatomical variations of the left subclavian artery and their significance in clinical practice

A Botou, E Panagouli, M Piagkou… - Journal of Long …, 2020 - dl.begellhouse.com
We study variations of the aortic arch (AA) and its first branches, the brachiocephalic, common
carotid, and subclavian arteries. Specifically, we describe anatomical variations of the left …

[HTML][HTML] Haemorrhage in pre-existing adrenal masses. A case series

AM Nixon, A Botou, C Aggeli, E Falidas… - International Journal of …, 2020 - Elsevier
Introduction Adrenal haemorrhage in the context of a pre-existing adrenal mass is a rare,
underestimated and potentially fatal surgical emergency. It is a rare cause of acute abdominal …