[HTML][HTML] Nanobody detection of standard fluorescent proteins enables multi-target DNA-PAINT with high resolution and minimal displacement errors
…, N Oleksiievets, S Isbaner, M Eggert-Martinez… - Cells, 2019 - mdpi.com
DNA point accumulation for imaging in nanoscale topography (PAINT) is a rapidly developing
fluorescence super-resolution technique, which allows for reaching spatial resolutions …
fluorescence super-resolution technique, which allows for reaching spatial resolutions …
Imaging minimal bacteria at the nanoscale: a reliable and versatile process to perform single-molecule localization microscopy in mycoplasmas
F Rideau, A Villa, P Belzanne, E Verdier… - Microbiology …, 2022 - Am Soc Microbiol
… Here, we describe an efficient and reliable protocol to perform single-molecule localization
microscopy (SMLM) imaging in mycoplasmas. We provide a polyvalent transposon-based …
microscopy (SMLM) imaging in mycoplasmas. We provide a polyvalent transposon-based …
[HTML][HTML] Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes
M Heilemann, S Van De Linde… - Angewandte Chemie …, 2008 - pub.uni-bielefeld.de
… ., Kasper, R., Seefeldt, B., Mukherjee, A., Tinnefeld, P., Sauer, M.: Subdiffraction-resolution
fluorescence imaging with conventional fluorescent probes. ANGEWANDTE CHEMIE-…
fluorescence imaging with conventional fluorescent probes. ANGEWANDTE CHEMIE-…
[HTML][HTML] A cross-nearest neighbor/Monte Carlo algorithm for single-molecule localization microscopy defines interactions between p53, Mdm2, and MEG3
NC Bauer, A Yang, X Wang, Y Zhou, A Klibanski… - Journal of Biological …, 2021 - ASBMB
The functions of long noncoding (lnc)RNAs, such as MEG3, are defined by their interactions
with other RNAs and proteins. These interactions, in turn, are shaped by their subcellular …
with other RNAs and proteins. These interactions, in turn, are shaped by their subcellular …
Small peptide–protein interaction pair for genetically encoded, fixation compatible peptide-PAINT
Super-resolution microscopy via PAINT has been widely adopted in life sciences to interrogate
the nanoscale architecture of many cellular structures. However, obtaining quantitative …
the nanoscale architecture of many cellular structures. However, obtaining quantitative …
Single-molecule fluorescence lifetime imaging using wide-field and confocal-laser scanning microscopy: a comparative analysis
N Oleksiievets, C Mathew, JC Thiele, JI Gallea… - Nano Letters, 2022 - ACS Publications
A recent addition to the toolbox of super-resolution microscopy methods is fluorescence-lifetime
single-molecule localization microscopy (FL-SMLM). The synergy of SMLM and …
single-molecule localization microscopy (FL-SMLM). The synergy of SMLM and …
Comparing divisome organization between vegetative and sporulating Bacillus subtilis at the nanoscale using DNA-PAINT
K Cramer, SCM Reinhardt, A Auer, JY Shin… - Science …, 2024 - science.org
Spore-forming bacteria have two distinct division modes: sporulation and vegetative division.
The placement of the foundational division machinery component (Z-ring) within the …
The placement of the foundational division machinery component (Z-ring) within the …
Mapping activity-dependent quasi-stationary states of mitochondrial membranes with graphene-induced energy transfer imaging
… We used 0.5 M trehalose, a nonreacting complex sugar for this purpose. Confocal
micrographs, as presented in Figure 4A and plots from line scans in Figure 4B, demonstrate the …
micrographs, as presented in Figure 4A and plots from line scans in Figure 4B, demonstrate the …
[HTML][HTML] Nanobody-based probes for subcellular protein identification and visualization
MA De Beer, BNG Giepmans - Frontiers in cellular neuroscience, 2020 - frontiersin.org
Understanding how building blocks of life contribute to physiology is greatly aided by protein
identification and cellular localization. The two main labeling approaches developed over …
identification and cellular localization. The two main labeling approaches developed over …
[HTML][HTML] A fluorescent probe for STED microscopy to study NIP-specific B cells
…, MAG de Castro, KM Yip, O Asadpour, M Münchhalfen… - Analyst, 2021 - pubs.rsc.org
We have developed a series of monovalent fluorophore-conjugated affinity probes based
on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model …
on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model …