… Mary Ellen Harper… Direct determinations of K m values for both AMP and ATP as well as
protein NMR evaluations could offer the most insight into the effect of these altered residues. …

The mitochondrial uncoupling protein (UCP) in the mitochondrial inner membrane of mammalian
brown adipose tissue generates heat by uncoupling oxidative phosphorylation 1 . This …

Glutathione (GSH) is the most abundant non-protein thiol present at millimolar concentrations
in mammalian tissues. As an important intracellular antioxidant, it acts as a regulator of …

Reactive oxygen species (ROS), natural by-products of aerobic respiration, are important
cell signaling molecules, which left unchecked can severely impair cellular functions and …

The yeast sir2 gene and its orthologues in Drosophila and C. elegans have well-established
roles in lifespan determination and response to caloric restriction. We have studied mice …

β 3 -Adrenergic receptors (β 3 -ARs) are expressed predominantly in white and brown adipose
tissue, and β 3 -selective agonists are potential anti-obesity drugs. However, the role of β …

Regulated mechanisms of stem cell maintenance are key to preventing stem cell depletion
and aging. While mitochondrial morphology plays a fundamental role in tissue development …

All nuclear-encoded mRNAs contain a 5′ cap structure (m 7 GpppN, where N is any nucleotide),
which is recognized by the eukaryotic translation initiation factor 4E (eIF4E) subunit of …

… in 250 mm sucrose, 5 mm Tris-HCl, and 1 mm EGTA (pH 7.4), and the final pellet was
resuspended in 120 mm KCl, 2 mmKH 2 PO 4 , 2 mm MgCl 2 , 1 mm EGTA, and 5 mm HEPES (pH …

Cristae, the organized invaginations of the mitochondrial inner membrane, respond structurally
to the energetic demands of the cell. The mechanism by which these dynamic changes …