User profiles for M. Isalan

Mark Isalan

Professor of Synthetic Biology, Imperial College London
Verified email at imperial.ac.uk
Cited by 4684

[HTML][HTML] Gene therapy advances: a meta-analysis of AAV usage in clinical settings

HKE Au, M Isalan, M Mielcarek - Frontiers in medicine, 2022 - frontiersin.org
Adeno-associated viruses (AAVs) are the safest and most effective gene delivery vehicles to
drive long-term transgene expression in gene therapy. While animal studies have shown …

Molecular biology of microbial ureases

HL Mobley, MD Island, RP Hausinger - Microbiological reviews, 1995 - Am Soc Microbiol
Urease (urea amidohydrolase; EC 3.5.1.5) catalyzes the hydrolysis of urea to yield
ammonia and carbamate. The latter compound spontaneously decomposes to yield another …

[HTML][HTML] A primer to directed evolution: current methodologies and future directions

LS Vidal, M Isalan, JT Heap… - RSC Chemical Biology, 2023 - pubs.rsc.org
Directed evolution is one of the most powerful tools for protein engineering and functions by
harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of …

Evolvability and hierarchy in rewired bacterial gene networks

M Isalan, C Lemerle, K Michalodimitrakis, C Horn… - Nature, 2008 - nature.com
Sequencing DNA from several organisms has revealed that duplication and drift of existing
genes have primarily moulded the contents of a given genome. Though the effect of knocking …

Zinc-finger protein-targeted gene regulation: genomewide single-gene specificity

…, L Reynolds, M Moore, M Isalan… - Proceedings of the …, 2003 - National Acad Sciences
Zinc-finger protein transcription factors (ZFP TFs) can be designed to control the expression
of any desired target gene, and thus provide potential therapeutic tools for the study and …

Synthetic zinc finger repressors reduce mutant huntingtin expression in the brain of R6/2 mice

…, M Dierssen, C Fillat, M Isalan - Proceedings of the …, 2012 - National Acad Sciences
Huntington's disease (HD) is a dominantly inherited neurodegenerative disorder caused by
expanded CAG repeats in the huntingtin (HTT) gene. Although several palliative treatments …

A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter

M Isalan, A Klug, Y Choo - Nature biotechnology, 2001 - nature.com
… Retained phage were eluted in 0.1 M triethylamine and neutralized with an equal volume
of 1 M Tris-HCl (pH 7.4). Logarithmic-phase E. coli TG1 were infected with eluted phage, and …

Synergy between adjacent zinc fingers in sequence-specific DNA recognition

M Isalan, Y Choo, A Klug - Proceedings of the National …, 1997 - National Acad Sciences
Zif268-like zinc fingers are generally regarded as independent DNA-binding modules that
each specify three base pairs in adjacent, but discrete, subsites. However, crystallographic …

[HTML][HTML] A unified design space of synthetic stripe-forming networks

…, M Gili, J Cotterell, J Sharpe, M Isalan - Nature …, 2014 - nature.com
Synthetic biology is a promising tool to study the function and properties of gene regulatory
networks. Gene circuits with predefined behaviours have been successfully built and …

Inhibition of herpes simplex virus 1 gene expression by designer zinc-finger transcription factors

M Papworth, M Moore, M Isalan… - Proceedings of the …, 2003 - National Acad Sciences
… selected directly from Lib23 by using the method described by Isalan and colleagues (12,
32). … M. Minczuk was also sponsored by European Molecular Biology Organization Short-Term …