At the beginning of mammalian life, the genetic material from each parent meets when the
fertilized egg divides. It was previously thought that a single microtubule spindle is …

The formation of mitotic chromosomes requires both compaction of chromatin and the
resolution of replicated sister chromatids. Compaction occurs during mitotic prophase and …

Self-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins
with synthetic fluorophores for use in fluorescence microscopy. Here we introduce HaloTag …

Chromosome organization in higher eukaryotes controls gene expression, DNA replication,
and DNA repair. Genome mapping has revealed the functional units of chromatin at the …

We assessed the intracellular association states of the Parkinson's disease related protein α-synuclein
(AS) in living cells by transfection with a functional recombinant mutant protein (…

Quantum dots multifunctionalized with the amyloid protein α-synuclein act at nanomolar
concentrations as very potent inducers of the aggregation of micromolar−millimolar bulk …

The morphological features of α-synuclein (AS) amyloid aggregation in vitro and in cells were
elucidated at the nanoscale by far-field subdiffraction fluorescence localization microscopy…

The Leica SP8 FALCON (FAst Lifetime CONtrast) is a fast and completely integrated
fluorescence lifetime imaging microscopy (FLIM) confocal platform. SP8 FALCON delivers video-…

A coumarin fluorophore and an oxazine photochrome can be integrated within the same
molecular skeleton and connected covalently to a secondary antibody. Illumination of the …

The advent of genome-wide RNA interference (RNAi)–based screens puts us in the position
to identify genes for all functions human cells carry out. However, for many functions, assay …