Here we describe a method for fabricating a primary human Small Intestine-on-a-Chip (Intestine
Chip) containing epithelial cells isolated from healthy regions of intestinal biopsies. The …

The blood-brain barrier (BBB) tightly regulates the entry of solutes from blood into the brain
and is disrupted in several neurological diseases. Using Organ-Chip technology, we created …

… osmium tetroxide in 0.1 M sodium cacodylate buffer, dehydrated in a series of graded
concentrations of ethanol solutions and critical point dried, as described previously (Kasendra et al.…

Background & Aims The mucus layer in the human colon protects against commensal
bacteria and pathogens, and defects in its unique bilayered structure contribute to intestinal …

Background Species-specific differences in tolerance to infection are exemplified by the high
susceptibility of humans to enterohemorrhagic Escherichia coli (EHEC) infection, whereas …

SslE is a zinc-metalloprotease involved in the degradation of mucin substrates and recently
proposed as a potential vaccine candidate against pathogenic E. coli. In this paper, by …

There is increasing evidence that the study of normal human enteroids duplicates many known
aspects of human intestinal physiology. However, this epithelial cell-only model lacks the …

… (M) List of biological processes identified by the GO enrichment analysis, using the
differentially expressed genes between the epithelial cells on day 8 of culture with endothelium, in …

Clostridium difficile is a cause of antibiotic-associated diarrhea and colitis, a healthcare-associated
intestinal disease. Colonization of the gut is a critical step in the course of infection. …

Modeling host-pathogen interactions with human intestinal epithelia using enteroid monolayers
on permeable supports (such as Transwells) represents an alternative to animal studies …