The ability of microorganisms to withstand long periods with extremely low energy input has
gained increasing scientific attention in recent years. Starvation experiments in the …

Betaine is an essential osmolyte and source of methyl groups and comes from either the diet
or by the oxidation of choline. Its metabolism methylates homocysteine to methionine, also …

In marine sediments, DNA occurs both inside and outside living organisms. DNA not enclosed
in living cells may account for the largest fraction of total DNA, and include molecules …

… Variants of this basic procedure were tried with 5 M K&O, instead of 0.5 M NaOH, and with …
p-hydroxybenzoic acid hydrazide (1%) in 0.5M NaOH and heated in a boiling water bath for 5 …

… The situation is not much different outside SMTZs; only two samples from 0.7 m below
seafloor at Sites 1227 and 1229 showed slightly higher bacterial than archaeal counts (Table 1). …

… Prokaryotic biomass in deep marine sediments exceeds 10 5 microbial cells/cm 3 even at
depths close to 1,000 m below the seafloor (mbsf) (1, 2). Extrapolation of these numbers to a …

… Ridge, is covered by a 265-m-thick sediment layer and lies ~2 … of the upper basement [265
to 350 m below seafloor (mbsf)], … the α subunit of methyl coenzyme M reductase (mcrA), a …

… reductase subunits A, B and C (hdrABC), methyl-viologen-reducing hydrogenase
subunits A, G and D (mvhAGD), and N5-methyltetrahydromethanopterin-coenzyme M …

… of NaCl in the presence of 0.1 M phosphate 11 . Under these conditions, the … M NaCl, the
H2A–H2B dimers elute at roughly 0.8 M NaCl, and the H3–H4 tetramers elute at around 1.2 M …

Microbial life inhabits deeply buried marine sediments, but the extent of this vast ecosystem
remains poorly constrained. Here we provide evidence for the existence of microbial …