… Briefly, cell were pelleted by mild centrifugation (200g) and fixed in 2.5% glutaraldehyde in
0.1 M sodium cacodylate buffer for 1 h at room temperature, post-fixed for 1 h in 1% aqueous …

Recent experimental and computational efforts have provided large data sets describing
three-dimensional organization of mouse and human genomes and showed the …

Chromosomes are hierarchically folded within cell nuclei into territories, domains and
subdomains, but the functional importance and evolutionary dynamics of these hierarchies are …

… Cells were treated with 0.075 M KCl hypotonic solution for 20 min at 37 C, collected by
centrifugation and fixed in cold 3:1 methanol-acetic acid solution according to the standard …

… The overall level of Kdr expression was significantly lower in the control experiment with
M. castaneus x M. musculus Wt hybrids, with both alleles being equally active. Thus, ectopic …

Motivation Recent development of Hi-C technique, a biochemical method to study 3D
genome architecture, provided large amount of information describing spatial organization of …

The article reveals some theoretical and practical aspects of the organization of independent
work of students, presents the results of studying general educational and methodological …

Abstract Properties of thermostable lipases from Geobacillus bacteria are considered. The
enzymes are divided into groups with regard to their thermostability. Coordinated amino acid …

Identifying structural variants (SVs) remains a pivotal challenge within genomic studies. The
recent advent of chromosome conformation capture (3C) techniques has emerged as a …

Effective molecular diagnosis of congenital diseases hinges on comprehensive genomic
analysis, traditionally reliant on various methodologies specific to each variant type - whole …