Recombineering, a recently developed technique for efficient genetic manipulation of bacteria,
is facilitated by phage-derived recombination proteins and has the advantage of using …

Probiotics have been shown to be effective against infectious diseases in clinical trials, with
either intestinal or extraintestinal health benefits. Even though probiotic effects are strain-…

Demand for new and novel natural compounds has intensified the development of plant-derived
compounds known as bioactives that either promote health or are toxic when ingested. …

Microbiomes are vast communities of microorganisms and viruses that populate all natural
ecosystems. Viruses have been considered to be the most variable component of …

Advances in DNA sequencing technology have facilitated the determination of hundreds of
complete genome sequences both for bacteria and their bacteriophages. Some of these …

Aims: To study the modification of the cell wall of Lactobacillus casei ATCC 393 grown in
high salt conditions. Methods and Results: Differences in the overall structure of cell wall …

… First, we examined the cellular localization of MSMEG3721 in M. smegmatis, because cell
wall enzymes are likely to be located on the exterior of the cell. To monitor the expression and …

… that we should be able to detect relatively small numbers of M. smegmatis bacteria. To
evaluate this we made dilutions of an M. smegmatis culture and infected 200 µl aliquots each with …

… bovis BCG, although we observed a reduction of efficiency of plating relative to M. … re-spotted
on lawns of M. marinum did not show an increased ability to infect either M. marinum strain …

Archaea, bacteria, and eukarya secrete membrane microvesicles (MVs) as a mechanism for
intercellular communication. We report the isolation and characterization of MVs from the …