New classification of Allium L. subg. Melanocrommyum (Webb & Berthel.) Rouy (Alliaceae) based on molecular and morphological characters.

RM Fritsch, FR Blattner, M Gurushidze - Phyton (Horn), 2010 - cabdirect.org
Allium subgenus Melanocrommyum from Eurasia is taxonomically complicated with different
and contradictory taxonomic treatments proposed in the past. A comprehensive study, …

Point mutation impairs centromeric CENH3 loading and induces haploid plants

…, N Stein, S Heckmann, M Gurushidze… - Proceedings of the …, 2015 - National Acad Sciences
The chromosomal position of the centromere-specific histone H3 variant CENH3 (also called
“CENP-A”) is the assembly site for the kinetochore complex of active centromeres. Any …

[HTML][HTML] True-breeding targeted gene knock-out in barley using designer TALE-nuclease in haploid cells

M Gurushidze, G Hensel, S Hiekel, S Schedel… - PloS one, 2014 - journals.plos.org
Transcription activator-like effector nucleases (TALENs) are customizable fusion proteins
able to cleave virtually any genomic DNA sequence of choice, and thereby to generate site-…

Targeted modification of gene function exploiting homology-directed repair of TALEN-mediated double-strand breaks in barley

…, T Rutten, M Gurushidze… - G3: Genes …, 2015 - academic.oup.com
Transcription activator-like effector nucleases open up new opportunities for targeted
mutagenesis in eukaryotic genomes. Similar to zinc-finger nucleases, sequence-specific DNA-…

Phylogenetic analysis of Allium subg. Melanocrommyum infers cryptic species and demands a new sectional classification

M Gurushidze, RM Fritsch, FR Blattner - Molecular Phylogenetics and …, 2008 - Elsevier
Allium subgenus Melanocrommyum (Alliaceae) from Eurasia comprises about 150 mostly
diploid species adapted to arid conditions. The group is taxonomically complicated with …

Species–level phylogeny of Allium subgenus Melanocrommyum: Incomplete lineage sorting, hybridization and trnF gene duplication

M Gurushidze, RM Fritsch, FR Blattner - Taxon, 2010 - Wiley Online Library
Noncoding chloroplast DNA sequences are widely employed markers in plant species–level
phylogenetics and phylogeography. However, chloroplast capture (hybridization) and …

[HTML][HTML] A simple test for the cleavage activity of customized endonucleases in plants

N Budhagatapalli, S Schedel, M Gurushidze, S Pencs… - Plant Methods, 2016 - Springer
Background Although customized endonucleases [transcription activator-like effector nucleases
(TALENs) and RNA-guided endonucleases (RGENs)] are known to be effective agents …

Genetic diversity of Vitis vinifera in Georgia: relationships between local cultivars and wild grapevine, V. vinifera L. subsp. sylvestris

J Ekhvaia, M Gurushidze, FR Blattner… - Genetic resources and …, 2014 - Springer
The Caucasus and Middle East regions are considered to be the primary centre of origin of
cultivated grapevine, and, as confirmed by archaeobotanical, archaeological, and cultural …

The evolution of genome size variation in drumstick onions (Allium subgenus Melanocrommyum)

M Gurushidze, J Fuchs, FR Blattner - Systematic Botany, 2012 - ingentaconnect.com
Allium subgenus Melanocrommyum is a species-rich group of perennial onions with
uniform karyology, ecology, and breeding system. Thus, the cytological, ecological, and …

[PDF][PDF] Site-directed mutagenesis in barley by expression of TALE nuclease in embryogenic pollen

M Gurushidze, S Hiekel, I Otto, G Hensel… - … for Plant Mutation …, 2017 - library.oapen.org
In this chapter, a detailed protocol for the induction of targeted mutations in barley using
customized transcription activator-like effector nucleases (TALENs) is provided. As explant for …