User profiles for Marc W. Kirschner
Marc KirschnerProfessor of Systems Biology, Harvard Medical School Verified email at hms.harvard.edu Cited by 137687 |
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
DW Cleveland, SG Fischer, MW Kirschner… - Journal of Biological …, 1977 - ASBMB
A rapid and convenient method for peptide mapping of proteins has been developed. The
technique, which is especially suitable for analysis of proteins that have been isolated from …
technique, which is especially suitable for analysis of proteins that have been isolated from …
Cyclin is degraded by the ubiquitin pathway
Cyclin degradation is the key step governing exit from mitosis and progress into the next cell
cycle. When a region in the N terminus of cyclin is fused to a foreign protein, it produces a …
cycle. When a region in the N terminus of cyclin is fused to a foreign protein, it produces a …
A protein factor essential for microtubule assembly.
…, SY Hwo, MW Kirschner - Proceedings of the …, 1975 - National Acad Sciences
A heat stable protein essentail for microtubule assembly has been isolated. This protein,
which we designate tau (tau), is present in association with tubulin purified from porcine brain …
which we designate tau (tau), is present in association with tubulin purified from porcine brain …
[PDF][PDF] Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells
It has long been the dream of biologists to map gene expression at the single-cell level.
With such data one might track heterogeneous cell sub-populations, and infer regulatory …
With such data one might track heterogeneous cell sub-populations, and infer regulatory …
Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling
…, S Ghidelli, S Fawell, C Lu, D Curtis, MW Kirschner… - Nature, 2009 - nature.com
The stability of the Wnt pathway transcription factor β-catenin is tightly regulated by the multi-subunit
destruction complex. Deregulated Wnt pathway activity has been implicated in many …
destruction complex. Deregulated Wnt pathway activity has been implicated in many …
Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS
…, J Rush, O Stemman, MW Kirschner… - Proceedings of the …, 2003 - National Acad Sciences
A need exists for technologies that permit the direct quantification of differences in protein
and posttranslationally modified protein expression levels. Here we present a strategy for the …
and posttranslationally modified protein expression levels. Here we present a strategy for the …
How proteolysis drives the cell cycle
Oscillations in the activity of cyclin-dependent kinases (CDKs) promote progression through
the eukaryotic cell cycle. This review examines how proteolysis regulates CDK activity—by …
the eukaryotic cell cycle. This review examines how proteolysis regulates CDK activity—by …
Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis
The caspase-3 (CPP32, apopain, YAMA) family of cysteinyl proteases has been implicated
as key mediators of apoptosis in mammalian cells. Gelsolin was identified as a substrate for …
as key mediators of apoptosis in mammalian cells. Gelsolin was identified as a substrate for …
[HTML][HTML] The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly
…, M Lopez, T Kirchhausen, T Takenawa, MW Kirschner - Cell, 1999 - cell.com
Although small GTP-binding proteins of the Rho family have been implicated in signaling to
the actin cytoskeleton, the exact nature of the linkage has remained obscure. We describe a …
the actin cytoskeleton, the exact nature of the linkage has remained obscure. We describe a …
Cyclin synthesis drives the early embryonic cell cycle
AW Murray, MW Kirschner - Nature, 1989 - nature.com
We have produced extracts of frog eggs that can perform multiple cell cycles in vitro. Destruction
of the endogenous messenger RNA arrests the extracts in interphase. The addition of …
of the endogenous messenger RNA arrests the extracts in interphase. The addition of …