In vivo CRISPR editing with no detectable genome-wide off-target mutations
CRISPR–Cas genome-editing nucleases hold substantial promise for developing human
therapeutic applications 1 , 2 , 3 , 4 , 5 – 6 but identifying unwanted off-target mutations is …
therapeutic applications 1 , 2 , 3 , 4 , 5 – 6 but identifying unwanted off-target mutations is …
[HTML][HTML] Harnessing DSB repair to promote efficient homology-dependent and-independent prime editing
…, S Šviković, G Sienski, M Firth, M Maresca - Nature …, 2022 - nature.com
Prime editing recently emerged as a next-generation approach for precise genome editing.
Here we exploit DNA double-strand break (DSB) repair to develop two strategies that install …
Here we exploit DNA double-strand break (DSB) repair to develop two strategies that install …
[HTML][HTML] Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery
…, R Nitsch, M Bohlooly-Y, ST Barry, M Maresca - Nature …, 2020 - nature.com
The CRISPR-Cas9 system has increased the speed and precision of genetic editing in cells
and animals. However, model generation for drug development is still expensive and time-…
and animals. However, model generation for drug development is still expensive and time-…
Obligate ligation-gated recombination (ObLiGaRe): custom-designed nuclease-mediated targeted integration through nonhomologous end joining
M Maresca, VG Lin, N Guo, Y Yang - Genome research, 2013 - genome.cshlp.org
Custom-designed nucleases (CDNs) greatly facilitate genetic engineering by generating a
targeted DNA double-strand break (DSB) in the genome. Once a DSB is created, specific …
targeted DNA double-strand break (DSB) in the genome. Once a DSB is created, specific …
Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq
…, HL Watry, LM Judge, BR Conklin, M Maresca… - Science, 2019 - science.org
CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target
sites. Current off-target discovery methods work using purified DNA or specific cellular models …
sites. Current off-target discovery methods work using purified DNA or specific cellular models …
[PDF][PDF] Functional gut microbiota remodeling contributes to the caloric restriction-induced metabolic improvements
…, D Rigo, S Leo, C Veyrat-Durebex, N Gaïa, M Maresca… - Cell metabolism, 2018 - cell.com
Caloric restriction (CR) stimulates development of functional beige fat and extends healthy
lifespan. Here we show that compositional and functional changes in the gut microbiota …
lifespan. Here we show that compositional and functional changes in the gut microbiota …
[HTML][HTML] Single-stranded heteroduplex intermediates in λ Red homologous recombination
Background The Red proteins of lambda phage mediate probably the simplest and most
efficient homologous recombination reactions yet described. However the mechanism of …
efficient homologous recombination reactions yet described. However the mechanism of …
Decoding non-random mutational signatures at Cas9 targeted sites
…, LM Mayr, M Bohlooly-Y, M Maresca - Nucleic acids …, 2018 - academic.oup.com
The mutation patterns at Cas9 targeted sites contain unique information regarding the nuclease
activity and repair mechanisms in mammalian cells. However, analytical framework for …
activity and repair mechanisms in mammalian cells. However, analytical framework for …
[HTML][HTML] In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model
Background Plasma concentration of low-density lipoprotein (LDL) cholesterol is a well-established
risk factor for cardiovascular disease. Inhibition of proprotein convertase subtilisin/…
risk factor for cardiovascular disease. Inhibition of proprotein convertase subtilisin/…
[HTML][HTML] Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editing
…, P Akcakaya, A Taheri-Ghahfarokhi, M Maresca - Nature …, 2023 - nature.com
Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical research
and offers potential cures for genetic diseases. Despite rapid progress, low efficiency of …
and offers potential cures for genetic diseases. Despite rapid progress, low efficiency of …