CRISPR–Cas genome-editing nucleases hold substantial promise for developing human
therapeutic applications 1 , 2 , 3 , 4 , 5 – 6 but identifying unwanted off-target mutations is …

Prime editing recently emerged as a next-generation approach for precise genome editing.
Here we exploit DNA double-strand break (DSB) repair to develop two strategies that install …

The CRISPR-Cas9 system has increased the speed and precision of genetic editing in cells
and animals. However, model generation for drug development is still expensive and time-…

Custom-designed nucleases (CDNs) greatly facilitate genetic engineering by generating a
targeted DNA double-strand break (DSB) in the genome. Once a DSB is created, specific …

CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target
sites. Current off-target discovery methods work using purified DNA or specific cellular models …

Caloric restriction (CR) stimulates development of functional beige fat and extends healthy
lifespan. Here we show that compositional and functional changes in the gut microbiota …

Background The Red proteins of lambda phage mediate probably the simplest and most
efficient homologous recombination reactions yet described. However the mechanism of …

The mutation patterns at Cas9 targeted sites contain unique information regarding the nuclease
activity and repair mechanisms in mammalian cells. However, analytical framework for …

Background Plasma concentration of low-density lipoprotein (LDL) cholesterol is a well-established
risk factor for cardiovascular disease. Inhibition of proprotein convertase subtilisin/…

Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical research
and offers potential cures for genetic diseases. Despite rapid progress, low efficiency of …