The nucleolus and other ribonucleoprotein (RNP) bodies are membrane-less organelles
that appear to assemble through phase separation of their molecular components. However, …

Mitochondria contain an autonomous and spatially segregated genome. The organizational
unit of their genome is the nucleoid, which consists of mitochondrial DNA (mtDNA) and …

Phase separation is emerging as a paradigm to explain the self-assembly and organization
of membraneless bodies in the cell. Recent advances show that this principle also extends …

Many intracellular membraneless organelles form via phase separation of intrinsically
disordered proteins (IDPs) or regions (IDRs). These include the Caenorhabditis elegans protein …

The size of a typical eukaryotic cell is of the order of ∼10 μm. However, some cell types grow
to very large sizes, including oocytes (immature eggs) of organisms from humans to starfish…

In live cells, phase separation is thought to organize macromolecules into membraneless
structures known as biomolecular condensates. Here, we reconstituted transcription in …

The nuclear chromatin structure confines the movement of large macromolecular complexes
to interchromatin corrals. Herpesvirus capsids of approximately 125 nm assemble in the …

The nucleolus is a prominent nuclear condensate that plays a central role in ribosome
biogenesis by facilitating the transcription and processing of nascent ribosomal RNA (rRNA). A …

Biomolecular condensates appear throughout the cell, serving many different biochemical
functions. We argue that condensate functionality is optimized when the interactions driving …

Recent advances in mass spectrometry (MS) have provided means for large-scale
phosphoproteomic profiling of specific tissues. Here, we report results from large-scale tandem MS […