User profiles for Mark A. Behlke
 | Mark BehlkeIntegrated DNA Technologies Verified email at idtdna.com Cited by 22531 |
Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy
RNA interference (RNAi) is the process of sequence-specific post-transcriptional gene
silencing triggered by double-stranded RNAs 1 , 2 , 3 . In attempts to identify RNAi triggers that …
silencing triggered by double-stranded RNAs 1 , 2 , 3 . In attempts to identify RNAi triggers that …
Identification of preexisting adaptive immunity to Cas9 proteins in humans
The CRISPR–Cas9 system is a powerful tool for genome editing, which allows the precise
modification of specific DNA sequences. Many efforts are underway to use the CRISPR–Cas9 …
modification of specific DNA sequences. Many efforts are underway to use the CRISPR–Cas9 …
[HTML][HTML] Progress towards in vivo use of siRNAs
MA Behlke - Molecular Therapy, 2006 - cell.com
RNA interference (RNAi) has become the method of choice to suppress gene expression in
vitro. It is also emerging as a powerful tool for in vivo research with over 90 studies published …
vitro. It is also emerging as a powerful tool for in vivo research with over 90 studies published …
A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells
Translation of the CRISPR–Cas9 system to human therapeutics holds high promise. However,
specificity remains a concern especially when modifying stem cell populations. We show …
specificity remains a concern especially when modifying stem cell populations. We show …
Functional polarity is introduced by Dicer processing of short substrate RNAs
Synthetic RNA duplexes that are substrates for Dicer are potent triggers of RNA interference
(RNAi). Blunt 27mer duplexes can be up to 100-fold more potent than traditional 21mer …
(RNAi). Blunt 27mer duplexes can be up to 100-fold more potent than traditional 21mer …
Chemical Modification of siRNAs for In Vivo Use
MA Behlke - Oligonucleotides, 2008 - liebertpub.com
Well over a hundred reports have been published describing use of synthetic small-interfering
RNAs (siRNAs) in animals. The majority of these reports employed unmodified RNA …
RNAs (siRNAs) in animals. The majority of these reports employed unmodified RNA …
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
…, D Friedmann-Morvinski, ZR Cohen, MA Behlke… - Science …, 2020 - science.org
Harnessing CRISPR-Cas9 technology for cancer therapeutics has been hampered by low
editing efficiency in tumors and potential toxicity of existing delivery systems. Here, we …
editing efficiency in tumors and potential toxicity of existing delivery systems. Here, we …
A structural basis for discriminating between self and nonself double-stranded RNAs in mammalian cells
…, P Serbinowski, R Hartmann, T Fujita, MA Behlke… - Nature …, 2006 - nature.com
Nonspecific effects triggered by small interfering RNAs (siRNAs) complicate the use of RNA
interference (RNAi) to specifically downregulate gene expression 1 , 2 , 3 , 4 , 5 . To uncover …
interference (RNAi) to specifically downregulate gene expression 1 , 2 , 3 , 4 , 5 . To uncover …
Analysis of microRNA turnover in mammalian cells following Dicer1 ablation
Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their
overall persistence in the cell following processing. Characterization of such persistence is …
overall persistence in the cell following processing. Characterization of such persistence is …
Cellular localization of long non-coding RNAs affects silencing by RNAi more than by antisense oligonucleotides
KA Lennox, MA Behlke - Nucleic acids research, 2016 - academic.oup.com
Thousands of long non-coding RNAs (lncRNAs) have been identified in mammalian cells.
Some have important functions and their dysregulation can contribute to a variety of disease …
Some have important functions and their dysregulation can contribute to a variety of disease …