We find that several key endogenous protein structures give rise to intense second-harmonic
generation (SHG)—nonabsorptive frequency doubling of an excitation laser line. Second-…

The interrelationships of the endoplasmic reticulum (ER), microtubules, and intermediate
filaments were studied in the peripheral regions of thin, spread fibroblasts, epithelial, and …

The mechanism by which Golgi membrane proteins are retained within the Golgi complex in
the midst of a continuous flow of protein and lipid is not yet understood. The diffusional …

A microneedle puncture of the fibroblast or sea urchin egg surface rapidly evokes a localized
exocytotic reaction that may be required for the rapid resealing that follows this breach in …

The endoplasmic reticulum (ER) often forms stacked membrane sheets, an arrangement that
is likely required to accommodate a maximum of membrane-bound polysomes for secretory …

The Golgi complex is a dynamic organelle engaged in both secretory and retrograde
membrane traffic. Here, we use green fluorescent protein–Golgi protein chimeras to study Golgi …

We have compared the dorsoventral development of hemichordates and chordates to
deduce the organization of their common ancestor, and hence to identify the evolutionary …

Certain fluorescent dyes, previously reported to localize mitochondria, when used at higher
concentrations also localize a continuous net-like structure in both living and glutaraldehyde-…

Chromosome capture by microtubules is widely accepted as the universal mechanism of
spindle assembly in dividing cells. However, the observed length of spindle microtubules and …

Vesicle-vesicle fusion initiated in cell cytoplasm by high Ca 2+ can rapidly erect large
membrane boundaries. These might be used as a ‘patch’ for resealing plasma membrane …