A golden gate modular cloning toolbox for plants
C Engler, M Youles, R Gruetzner, TM Ehnert… - ACS synthetic …, 2014 - ACS Publications
Plant Synthetic Biology requires robust and efficient methods for assembling multigene
constructs. Golden Gate cloning provides a precision module-based cloning technique for facile …
constructs. Golden Gate cloning provides a precision module-based cloning technique for facile …
Standards for plant synthetic biology: a common syntax for exchange of DNA parts
…, H Warzecha, C Matthewman, M Youles… - New …, 2015 - Wiley Online Library
Inventors in the field of mechanical and electronic engineering can access multitudes of
components and, thanks to standardization, parts from different manufacturers can be used in …
components and, thanks to standardization, parts from different manufacturers can be used in …
Structural basis of hepatocyte growth factor/scatter factor and MET signalling
…, MV Petoukhov, J Finch, ME Youles… - Proceedings of the …, 2006 - National Acad Sciences
The polypeptide growth factor, hepatocyte growth factor/scatter factor (HGF/SF), shares the
multidomain structure and proteolytic mechanism of activation of plasminogen and other …
multidomain structure and proteolytic mechanism of activation of plasminogen and other …
Functional map and domain structure of MET, the product of the c-met protooncogene and receptor for hepatocyte growth factor/scatter factor
E Gherardi, ME Youles, RN Miguel… - Proceedings of the …, 2003 - National Acad Sciences
Little is known about the large ectodomain of MET, the product of the c-met protooncogene
and receptor for hepatocyte growth factor/scatter factor (HGF/SF). Here, we establish by …
and receptor for hepatocyte growth factor/scatter factor (HGF/SF). Here, we establish by …
Allelic compatibility in plant immune receptors facilitates engineering of new effector recognition specificities
…, CEM Stevenson, JHR Maidment, M Youles… - The Plant …, 2023 - academic.oup.com
Engineering the plant immune system offers genetic solutions to mitigate crop diseases
caused by diverse agriculturally significant pathogens and pests. Modification of intracellular …
caused by diverse agriculturally significant pathogens and pests. Modification of intracellular …
Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity
Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important
roles in recognition of pathogen-derived effectors. However, the mechanism by which plant …
roles in recognition of pathogen-derived effectors. However, the mechanism by which plant …
Engineering the NK1 fragment of hepatocyte growth factor/scatter factor as a MET receptor antagonist
M Youles, O Holmes, MV Petoukhov… - Journal of molecular …, 2008 - Elsevier
The growth and motility factor hepatocyte growth factor/scatter factor (HGF/SF) and its
receptor MET, the tyrosine kinase encoded by the c-MET proto-oncogene, exert major roles in …
receptor MET, the tyrosine kinase encoded by the c-MET proto-oncogene, exert major roles in …
pOPIN-GG: a resource for modular assembly in protein expression vectors
AR Bentham, M Youles, MN Mendel, FA Varden… - BioRxiv, 2021 - biorxiv.org
The ability to recombinantly produce target proteins is essential to many biochemical,
structural, and biophysical assays that allow for interrogation of molecular mechanisms behind …
structural, and biophysical assays that allow for interrogation of molecular mechanisms behind …
Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems
T Lawrenson, M Youles, M Chhetry, M Clarke… - … : Methods and Protocols, 2023 - Springer
CRISPR/Cas has been established for targeted mutagenesis in many plant species since
2013, including Brassica napus and Brassica oleracea. Since that time, improvements have …
2013, including Brassica napus and Brassica oleracea. Since that time, improvements have …
pOPARA: Vectors for Golden Gate assembly of expression constructs containing the araBAD promoter
L Milnes, M Youles, JHR Maidment - bioRxiv, 2023 - biorxiv.org
Heterologous protein production is often required to investigate the structural, biochemical,
and biophysical properties of a protein of interest. Frequently, optimisation of expression …
and biophysical properties of a protein of interest. Frequently, optimisation of expression …