A golden gate modular cloning toolbox for plants

C Engler, M Youles, R Gruetzner, TM Ehnert… - ACS synthetic …, 2014 - ACS Publications
Plant Synthetic Biology requires robust and efficient methods for assembling multigene
constructs. Golden Gate cloning provides a precision module-based cloning technique for facile …

Standards for plant synthetic biology: a common syntax for exchange of DNA parts

…, H Warzecha, C Matthewman, M Youles… - New …, 2015 - Wiley Online Library
Inventors in the field of mechanical and electronic engineering can access multitudes of
components and, thanks to standardization, parts from different manufacturers can be used in …

Structural basis of hepatocyte growth factor/scatter factor and MET signalling

…, MV Petoukhov, J Finch, ME Youles… - Proceedings of the …, 2006 - National Acad Sciences
The polypeptide growth factor, hepatocyte growth factor/scatter factor (HGF/SF), shares the
multidomain structure and proteolytic mechanism of activation of plasminogen and other …

Functional map and domain structure of MET, the product of the c-met protooncogene and receptor for hepatocyte growth factor/scatter factor

E Gherardi, ME Youles, RN Miguel… - Proceedings of the …, 2003 - National Acad Sciences
Little is known about the large ectodomain of MET, the product of the c-met protooncogene
and receptor for hepatocyte growth factor/scatter factor (HGF/SF). Here, we establish by …

Allelic compatibility in plant immune receptors facilitates engineering of new effector recognition specificities

…, CEM Stevenson, JHR Maidment, M Youles… - The Plant …, 2023 - academic.oup.com
Engineering the plant immune system offers genetic solutions to mitigate crop diseases
caused by diverse agriculturally significant pathogens and pests. Modification of intracellular …

Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity

…, A Redkar, H Brown, Y Ma, M Youles… - Journal of …, 2020 - academic.oup.com
Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important
roles in recognition of pathogen-derived effectors. However, the mechanism by which plant …

Engineering the NK1 fragment of hepatocyte growth factor/scatter factor as a MET receptor antagonist

M Youles, O Holmes, MV Petoukhov… - Journal of molecular …, 2008 - Elsevier
The growth and motility factor hepatocyte growth factor/scatter factor (HGF/SF) and its
receptor MET, the tyrosine kinase encoded by the c-MET proto-oncogene, exert major roles in …

pOPIN-GG: a resource for modular assembly in protein expression vectors

AR Bentham, M Youles, MN Mendel, FA Varden… - BioRxiv, 2021 - biorxiv.org
The ability to recombinantly produce target proteins is essential to many biochemical,
structural, and biophysical assays that allow for interrogation of molecular mechanisms behind …

Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems

T Lawrenson, M Youles, M Chhetry, M Clarke… - … : Methods and Protocols, 2023 - Springer
CRISPR/Cas has been established for targeted mutagenesis in many plant species since
2013, including Brassica napus and Brassica oleracea. Since that time, improvements have …

pOPARA: Vectors for Golden Gate assembly of expression constructs containing the araBAD promoter

L Milnes, M Youles, JHR Maidment - bioRxiv, 2023 - biorxiv.org
Heterologous protein production is often required to investigate the structural, biochemical,
and biophysical properties of a protein of interest. Frequently, optimisation of expression …