… Author links open overlay panel Marko Sustarsic , Achillefs N Kapanidis … Marko
Sustarsic was supported by The Welcome Trust; Achillefs Kapanidis was supported by a UK …

Membrane protein biogenesis in bacteria occurs via dedicated molecular systems SecYEG
and YidC that function independently and in cooperation. YidC belongs to the universally …

DNA-binding proteins utilise different recognition mechanisms to locate their DNA targets;
some proteins recognise specific DNA sequences, while others interact with specific DNA …

Studying the structure and dynamics of proteins in live cells is essential to understanding
their physiological activities and mechanisms, and to validating in vitro characterization. …

Herein we investigate the molecular bases of DNA polymerase I conformational dynamics
that underlie the replication fidelity of the enzyme. Such fidelity is determined by …

The ability to study biomolecules in vivo is crucial for understanding their function in a
biological context. One powerful approach involves fusing molecules of interest to fluorescent …

DNA-binding proteins utilise different recognition mechanisms to locate their DNA targets.
Some proteins recognise specific nucleotide sequences, while many DNA repair proteins …

DNA polymerases are a family of molecular machines involved in high-fidelity DNA replication
and repair, of which DNA polymerase I (Pol) is one the best-characterized members. Pol …

458a Tuesday, February 18, 2014 and thoroughly washed to remove any non-internalized
fluorescence. Internalized molecules can be tracked at the single-molecule level, and both …

DNA polymerase I Klenow Fragment (Pol) adopts multiple conformations during high-fidelity
DNA replication and repair, many of which have not been resolved by conventional …