High-throughput single-cell RNA sequencing has transformed our understanding of complex
cell populations, but it does not provide phenotypic information such as cell-surface protein …

Single-cell transcriptomics has transformed our ability to characterize cell states, but deep
biological understanding requires more than a taxonomic listing of clusters. As new methods …

Despite rapid developments in single cell sequencing, sample-specific batch effects,
detection of cell multiplets, and experimental costs remain outstanding challenges. Here, we …

The simultaneous measurement of multiple modalities represents an exciting frontier for
single-cell genomics and necessitates computational methods that can define cellular states …

The mutualistic relationship of gut-resident microbiota and the host immune system promotes
homeostasis that ensures maintenance of the microbial community and of a largely non-…

The global rise in obesity has revitalized a search for genetic and epigenetic factors underlying
the disease. We present a Drosophila model of paternal-diet-induced intergenerational …

Multimodal single-cell assays provide high-resolution snapshots of complex cell populations,
but are mostly limited to transcriptome plus an additional modality. Here, we describe …

Spatial transcriptomics and proteomics provide complementary information that independently
transformed our understanding of complex biological processes. However, experimental …

Animal mRNAs are regulated by hundreds of RNA binding proteins (RBPs). The identification
of RBP targets is crucial for understanding their function. A recent method, PAR-CLIP, uses …

Developmental origins of dendritic cells (DCs) including conventional DCs (cDCs, comprising
cDC1 and cDC2 subsets) and plasmacytoid DCs (pDCs) remain unclear. We studied DC …