Fatty acids (FAs) are considered strategically important platform compounds that can be
accessed by sustainable microbial approaches. Here we report the reprogramming of chain-…

Fatty acids are among the major building blocks of living cells, making lipid biosynthesis a
potent target for compounds with antibiotic or antineoplastic properties. We present the crystal …

Fungal type I fatty acid synthases (FASs) are mega-enzymes with two separated, identical
compartments, in which the acyl carrier protein (ACP) domains shuttle substrates to …

The yeast fatty acid synthase (FAS) is a barrel-shaped 2.6 MDa complex. Upon barrel-formation,
two multidomain subunits, each more than 200 kDa large, intertwine to form a …

10.7554/eLife.42747.001 Electron cryo-microscopy analyzes the structure of proteins and
protein complexes in vitrified solution. Proteins tend to adsorb to the air-water interface in …

Fatty acid and polyketide synthases (FASs and PKSs) synthesize physiologically and
pharmaceutically important products by condensation of acyl building blocks. The transacylation …

Modular polyketide synthases (PKSs) run catalytic reactions over dozens of steps in a highly
orchestrated manner. To accomplish this synthetic feat, they form megadalton multienzyme …

Methanol is an ideal feedstock for chemical and biological manufacturing. Constructing an
efficient cell factory is essential for producing complex compounds through methanol …

Covering: mid 1990s to 2018 Over the last two decades, diverse approaches have been
explored to generate new polyketides by engineering polyketide synthases (PKSs). Although it …

De novo biosynthesis of fatty acids is an iterative process requiring strict regulation of the
lengths of the produced fatty acids. In this review, we focus on the factors determining chain …