RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts
JD Keene, JM Komisarow, MB Friedersdorf - Nature protocols, 2006 - nature.com
RNA targets of multitargeted RNA-binding proteins (RBPs) can be studied by various methods
including mobility shift assays, iterative in vitro selection techniques and computational …
including mobility shift assays, iterative in vitro selection techniques and computational …
[HTML][HTML] Advancing the functional utility of PAR-CLIP by quantifying background binding to mRNAs and lncRNAs
MB Friedersdorf, JD Keene - Genome biology, 2014 - Springer
Background Sequence specific RNA binding proteins are important regulators of gene
expression. Several related crosslinking-based, high-throughput sequencing methods, including …
expression. Several related crosslinking-based, high-throughput sequencing methods, including …
Coordinated posttranscriptional mRNA population dynamics during T‐cell activation
N Mukherjee, PJ Lager, MB Friedersdorf… - Molecular systems …, 2009 - embopress.org
Although RNA‐binding proteins (RBPs) coordinate many key decisions during cell growth
and differentiation, the dynamics of RNA–RBP interactions have not been extensively studied …
and differentiation, the dynamics of RNA–RBP interactions have not been extensively studied …
The RNA-binding protein DND1 acts sequentially as a negative regulator of pluripotency and a positive regulator of epigenetic modifiers required for germ cell …
VA Ruthig, MB Friedersdorf, JA Garness… - …, 2019 - journals.biologists.com
The adult spermatogonial stem cell population arises from pluripotent primordial germ cells (PGCs)
that enter the fetal testis around embryonic day (E)10.5. PGCs undergo rapid mitotic …
that enter the fetal testis around embryonic day (E)10.5. PGCs undergo rapid mitotic …
A transgenic DND1GFP fusion allele reports in vivo expression and RNA-binding targets in undifferentiated mouse germ cells
VA Ruthig, T Yokonishi, MB Friedersdorf… - Biology of …, 2021 - academic.oup.com
In vertebrates, the RNA-binding protein (RBP) dead end 1 (DND1) is essential for primordial
germ cell (PGC) survival and maintenance of cell identity. In multiple species, Dnd1 loss or …
germ cell (PGC) survival and maintenance of cell identity. In multiple species, Dnd1 loss or …
DO-RIP-seq to quantify RNA binding sites transcriptome-wide
CO Nicholson, MB Friedersdorf, LS Bisogno, JD Keene - Methods, 2017 - Elsevier
Post-transcriptional processes orchestrate gene expression through dynamic protein-RNA
interactions. These interactions occur at specific sites determined by RNA sequence, …
interactions. These interactions occur at specific sites determined by RNA sequence, …
[HTML][HTML] The RNA binding protein DND1 is elevated in a subpopulation of pro-spermatogonia and targets chromatin modifiers and translational machinery during late …
VA Ruthig, T Hatkevich, J Hardy, MB Friedersdorf… - PLoS …, 2023 - journals.plos.org
DND1 is essential to maintain germ cell identity. Loss of Dnd1 function results in germ cell
differentiation to teratomas in some inbred strains of mice or to somatic fates in zebrafish. …
differentiation to teratomas in some inbred strains of mice or to somatic fates in zebrafish. …
[PDF][PDF] Ras post-transcriptionally enhances a pre-malignantly primed EMT to promote invasion
LS Bisogno, MB Friedersdorf, JD Keene - Iscience, 2018 - cell.com
Epithelial-to-mesenchymal transition (EMT) is integral to cancer progression, with considerable
evidence that EMT has multiple intermediary stages. Understanding the mechanisms of …
evidence that EMT has multiple intermediary stages. Understanding the mechanisms of …
Development of novel eIF4E inhibitors to potently and selectively suppress tumor growth across multiple indications
MB Friedersdorf, DR Blake, SE Thompson, R Pavana… - Cancer Research, 2024 - AACR
Despite considerable progress in treating cancer, drug resistance remains the primary hurdle
to achieving cures in patients. Tumors often develop resistance through reactivation of the …
to achieving cures in patients. Tumors often develop resistance through reactivation of the …