Precommitment low-level Neurog3 expression defines a long-lived mitotic endocrine-biased progenitor pool that drives production of endocrine-committed cells
ME Bechard, ED Bankaitis, SB Hipkens… - Genes & …, 2016 - genesdev.cshlp.org
The current model for endocrine cell specification in the pancreas invokes high-level production
of the transcription factor Neurogenin 3 (Neurog3) in Sox9 + bipotent epithelial cells as …
of the transcription factor Neurogenin 3 (Neurog3) in Sox9 + bipotent epithelial cells as …
Feedback control of growth, differentiation, and morphogenesis of pancreatic endocrine progenitors in an epithelial plexus niche
ED Bankaitis, ME Bechard… - Genes & development, 2015 - genesdev.cshlp.org
In the mammalian pancreas, endocrine cells undergo lineage allocation upon emergence
from a bipotent duct/endocrine progenitor pool, which resides in the “trunk epithelium.” Major …
from a bipotent duct/endocrine progenitor pool, which resides in the “trunk epithelium.” Major …
EGFR signalling controls cellular fate and pancreatic organogenesis by regulating apicobasal polarity
…, P Nyeng, ME Bechard, K Hess, E Bankaitis… - Nature cell …, 2017 - nature.com
Apicobasal polarity is known to affect epithelial morphogenesis and cell differentiation, but it
remains unknown how these processes are mechanistically orchestrated. We find that ligand…
remains unknown how these processes are mechanistically orchestrated. We find that ligand…
[HTML][HTML] Pancreatic cancers suppress negative feedback of glucose transport to reprogram chromatin for metastasis
ME Bechard, R Smalling, A Hayashi, Y Zhong… - Nature …, 2020 - nature.com
Although metastasis is the most common cause of cancer deaths, metastasis-intrinsic
dependencies remain largely uncharacterized. We previously reported that metastatic pancreatic …
dependencies remain largely uncharacterized. We previously reported that metastatic pancreatic …
ROCK-nmMyoII, Notch and Neurog3 gene-dosage link epithelial morphogenesis with cell fate in the pancreatic endocrine-progenitor niche
ED Bankaitis, ME Bechard, G Gu… - …, 2018 - journals.biologists.com
During mouse pancreas organogenesis, endocrine cells are born from progenitors residing
in an epithelial plexus niche. After a period in a lineage-primed Neurog3 LO state, …
in an epithelial plexus niche. After a period in a lineage-primed Neurog3 LO state, …
Pentose conversions support the tumorigenesis of pancreatic cancer distant metastases
ME Bechard, AE Word, AV Tran, X Liu, JW Locasale… - Oncogene, 2018 - nature.com
Pancreatic ductal adenocarcinoma (PDAC) adopts several unique metabolic strategies to
support primary tumor growth. Whether additional metabolic strategies are adopted to support …
support primary tumor growth. Whether additional metabolic strategies are adopted to support …
[HTML][HTML] Obtaining patient-derived cancer organoid cultures via fine-needle aspiration
CJ Phifer, KN Bergdorf, ME Bechard, A Vilgelm… - STAR protocols, 2021 - Elsevier
Patient-derived tumor organoid cultures are an essential and innovative methodology for
translational research. However, current techniques to establish these cultures are …
translational research. However, current techniques to establish these cultures are …
Aminopyridine analogs selectively target metastatic pancreatic cancer
RV Smalling, ME Bechard, J Duryea, PJ Kingsley… - Oncogene, 2022 - nature.com
Metastatic outgrowth is supported by metabolic adaptations that may differ from the primary
tumor of origin. However, it is unknown if such adaptations are therapeutically actionable. …
tumor of origin. However, it is unknown if such adaptations are therapeutically actionable. …
[HTML][HTML] Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids
…, CJ Phifer, ME Bechard, MA Lee, OG McDonald, E Lee… - STAR protocols, 2021 - Elsevier
… Phifer 2 6 7 , Matthew E. Bechard 2 , Mason A. Lee 2 3 , Oliver G. McDonald 2 4 5 , Ethan
Lee 1 3 4 , Vivian L. … McDonald, E. Lee, V. Weiss …
Lee 1 3 4 , Vivian L. … McDonald, E. Lee, V. Weiss …
Application of a colorimetric assay to identify putative ribofuranosylaminobenzene 5′-phosphate synthase genes expressed with activity inEscherichia coli
ME Bechard, S Chhatwal, RE Garcia… - Biological procedures …, 2003 - Springer
Tetrahydromethanopterin (H 4 MPT) is a tetrahydrofolate analog originally discovered in
methanogenic archaea, but later found in other archaea and bacteria. The extent to which …
methanogenic archaea, but later found in other archaea and bacteria. The extent to which …