The coronavirus disease 2019 (COVID-19) pandemic caused by the SARS-CoV-2 (severe
acute respiratory syndrome coronavirus 2) coronavirus is a major public health challenge. …

The functional state of a cell is largely determined by the spatiotemporal organization of its
proteome. Technologies exist for measuring particular aspects of protein turnover and …

The nuclear envelope is a double membrane that separates the nucleus from the cytoplasm.
The inner nuclear membrane (INM) functions in essential nuclear processes including …

The yeast Saccharomyces cerevisiae is ideal for systematic studies relying on collections of
modified strains (libraries). Despite the significance of yeast libraries and the immense …

Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for …

Tandem fluorescent protein timers (tFTs) report on protein age through time-dependent
change in color, which can be exploited to study protein turnover and trafficking. Each tFT, …

Selective protein degradation by the ubiquitin-proteasome system (UPS) is involved in all
cellular processes. However, the substrates and specificity of most UPS components are not …

Hydrogen peroxide (H 2 O 2 ) sensing and signaling involves the reversible oxidation of
particular thiols on particular proteins to modulate protein function in a dynamic manner. H 2 O 2 …

Background To achieve higher effectiveness in population-based SARS-CoV-2 surveillance
and to reliably predict the course of an outbreak, screening, and monitoring of infected …

Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae
open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each …