BRCA1 deficiencies cause breast, ovarian, prostate and other cancers, and render tumours
hypersensitive to poly(ADP-ribose) polymerase (PARP) inhibitors. To understand the …

Mutations in the ATM tumor suppressor gene confer hypersensitivity to DNA-damaging
chemotherapeutic agents. To explore genetic resistance mechanisms, we performed genome-…

The activities of many DNA-repair proteins are controlled through reversible covalent
modification by ubiquitin and ubiquitin-like molecules. Nonhomologous end-joining (NHEJ) is the …

Covalent DNA–protein cross-links (DPCs) are toxic DNA lesions that block replication and
require repair by multiple pathways. Whether transcription blockage contributes to the toxicity …

Ubiquitylation is crucial for proper cellular responses to DNA double-strand breaks (DSBs).
If unrepaired, these highly cytotoxic lesions cause genome instability, tumorigenesis, …

CRISPR-Cas9 genome engineering has revolutionised high-throughput functional genomic
screens. However, recent work has raised concerns regarding the performance of CRISPR-…

Histone H2AX and MDC1 are key DNA repair and DNA-damage signalling proteins. When
DNA double-strand breaks (DSBs) occur, H2AX is phosphorylated and then recruits MDC1, …

The nucleoside decitabine (5-aza-dC) is used to treat several hematological cancers. Upon
triphosphorylation and incorporation into DNA, 5-aza-dC induces covalent DNMT1 DNA-…

Covalent DNA-protein crosslinks (DPCs) are toxic DNA lesions that block replication and
require repair by multiple pathways. Whether transcription blockage contributes to the toxicity of …

Complete set of graphs for gammaH2AX, 53BP1 and ubiquitin (FK2 antibody) foci/cell kinetics
in response to ionising radiation (IR)-and mock-treated human U2OS cells treated with …