[HTML][HTML] A catalogue of biochemically diverse CRISPR-Cas9 orthologs
…, G Bigelyte, JL Curcuru, M Mabuchi… - Nature …, 2020 - nature.com
Bacterial Cas9 nucleases from type II CRISPR-Cas antiviral defence systems have been
repurposed as genome editing tools. Although these proteins are found in many microbes, only …
repurposed as genome editing tools. Although these proteins are found in many microbes, only …
Precise DNA cleavage using CRISPR-SpRYgests
Methods for in vitro DNA cleavage and molecular cloning remain unable to precisely cleave
DNA directly adjacent to bases of interest. Restriction enzymes (REs) must bind specific …
DNA directly adjacent to bases of interest. Restriction enzymes (REs) must bind specific …
Staphylococcus aureus Cas9 is a multiple-turnover enzyme
P Yourik, RT Fuchs, M Mabuchi, JL Curcuru, GB Robb - Rna, 2019 - rnajournal.cshlp.org
Cas9 nuclease is the key effector of type II CRISPR adaptive immune systems found in
bacteria. The nuclease can be programmed by a single guide RNA (sgRNA) to cleave DNA in a …
bacteria. The nuclease can be programmed by a single guide RNA (sgRNA) to cleave DNA in a …
Target-dependent nickase activities of the CRISPR–Cas nucleases Cpf1 and Cas9
BXH Fu, JD Smith, RT Fuchs, M Mabuchi… - Nature …, 2019 - nature.com
Clustered regularly interspaced short palindromic repeats (CRISPR) machineries are
prokaryotic immune systems that have been adapted as versatile gene editing and manipulation …
prokaryotic immune systems that have been adapted as versatile gene editing and manipulation …
Cas12a trans-cleavage can be modulated in vitro and is active on ssDNA, dsDNA, and RNA
RT Fuchs, J Curcuru, M Mabuchi, P Yourik, GB Robb - BioRxiv, 2019 - biorxiv.org
CRISPR-Cas12a (Cpf1) are RNA-guided nuclease effectors of acquired immune response
that act in their native organisms by cleaving targeted DNA sequences. Like CRISPR-Cas9 …
that act in their native organisms by cleaving targeted DNA sequences. Like CRISPR-Cas9 …
A new family of CRISPR‐type V nucleases with C‐rich PAM recognition
…, M Stitilyte, M Jasnauskaite, M Mabuchi… - EMBO …, 2022 - embopress.org
Most CRISPR‐type V nucleases are stimulated to cleave double‐stranded (ds) DNA targets
by a T‐rich PAM, which restricts their targeting range. Here, we identify and characterize a …
by a T‐rich PAM, which restricts their targeting range. Here, we identify and characterize a …
DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavage
The RNA-guided Cas9 endonuclease from Staphylococcus aureus (SauCas9) can catalyze
multiple-turnover reactions whereas Cas9 from Streptococcus pyogenes (SpyCas9) is a …
multiple-turnover reactions whereas Cas9 from Streptococcus pyogenes (SpyCas9) is a …
[PDF][PDF] Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells
RNA-targeting CRISPR-Cas13 proteins have recently emerged as a powerful platform to
modulate gene expression outcomes. However, protein and CRISPR RNA (crRNA) delivery in …
modulate gene expression outcomes. However, protein and CRISPR RNA (crRNA) delivery in …
[HTML][HTML] Characterization of Cme and Yme thermostable Cas12a orthologs
RT Fuchs, JL Curcuru, M Mabuchi, A Noireterre… - Communications …, 2022 - nature.com
CRISPR-Cas12a proteins are RNA-guided endonucleases that cleave invading DNA
containing target sequences adjacent to protospacer adjacent motifs (PAM). Cas12a orthologs …
containing target sequences adjacent to protospacer adjacent motifs (PAM). Cas12a orthologs …
Structure and cleavage activity of the tetrameric MspJI DNA modification-dependent restriction endonuclease
JR Horton, MY Mabuchi, D Cohen-Karni… - Nucleic acids …, 2012 - academic.oup.com
The MspJI modification-dependent restriction endonuclease recognizes 5-methylcytosine or
5-hydroxymethylcytosine in the context of CNN(G/A) and cleaves both strands at fixed …
5-hydroxymethylcytosine in the context of CNN(G/A) and cleaves both strands at fixed …