Inducible depletion of PI(4,5)P2 by the synthetic iDePP system in Arabidopsis

M Doumane, A Lebecq, L Colin, A Fangain… - Nature Plants, 2021 - nature.com
Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P 2 ) is a low-abundance membrane lipid
essential for plasma membrane function 1 , 2 . In plants, mutations in phosphatidylinositol 4-…

Oomycete interactions with plants: infection strategies and resistance principles

S Fawke, M Doumane, S Schornack - Microbiology and Molecular …, 2015 - Am Soc Microbiol
The Oomycota include many economically significant microbial pathogens of crop species.
Understanding the mechanisms by which oomycetes infect plants and identifying methods to …

The Arabidopsis SAC9 enzyme is enriched in a cortical population of early endosomes and restricts PI (4, 5) P2 at the plasma membrane

A Lebecq, M Doumane, A Fangain, V Bayle, JX Leong… - Elife, 2022 - elifesciences.org
Membrane lipids, and especially phosphoinositides, are differentially enriched within the
eukaryotic endomembrane system. This generates a landmark code by modulating the …

Experimental manipulation of phosphoinositide lipids: from cells to organisms

M Doumane, MC Caillaud, Y Jaillais - Trends in Cell Biology, 2022 - cell.com
Phosphoinositides (PIs) have critical roles in various cellular, physiological, developmental,
pathological, and infectious processes. They are signaling phospholipids that can affect …

[PDF][PDF] A combinatorial lipid code shapes the electrostatic landscape of plant endomembranes

MP Platre, LC Noack, M Doumane, V Bayle… - Developmental cell, 2018 - cell.com
Membrane surface charge is critical for the transient, yet specific recruitment of proteins with
polybasic regions to certain organelles. In eukaryotes, the plasma membrane (PM) is the …

Arabidopsis ADR1 helper NLR immune receptors localize and function at the plasma membrane in a phospholipid dependent manner

…, A Bayless, V Bonardi, L Wan, M Doumane… - New …, 2021 - Wiley Online Library
Activation of nucleotide‐binding leucine‐rich repeat receptors (NLRs) results in immunity and
a localized cell death. NLR cell death activity requires oligomerization and in some cases …

[HTML][HTML] Time-resolved dual transcriptomics reveal early induced Nicotiana benthamiana root genes and conserved infection-promoting Phytophthora palmivora …

E Evangelisti, A Gogleva, T Hainaux, M Doumane… - BMC biology, 2017 - Springer
Background Plant-pathogenic oomycetes are responsible for economically important losses
in crops worldwide. Phytophthora palmivora, a tropical relative of the potato late blight …

Assessing Extrinsic Membrane Protein Dependency to PI4P Using a Plasma Membrane to Endosome Relocalization Transient Assay in Nicotiana benthamiana

M Doumane, MC Caillaud - Plant Endosomes: Methods and Protocols, 2020 - Springer
Phosphoinositides are key players from which the various membranes of the cells acquire
their identity. The relative accumulation of these low-abundant anionic phospholipids in the …

Automated tracking of root for confocal time-lapse imaging of cellular processes

M Doumane, C Lionnet, V Bayle, Y Jaillais… - Bio …, 2017 - bio-protocol.org
Here we describe a protocol that enables to automatically perform time-lapse imaging of
growing root tips for several hours. Plants roots expressing fluorescent proteins or stained with …

Coiled-coil and RPW8-type immune receptors function at the plasma membrane in a phospholipid dependent manner

…, A Bayless, E Stöbbe, V Bonardi, L Wan, M Doumane… - bioRxiv, 2020 - biorxiv.org
Activation of intracellular nucleotide-binding leucine-rich repeat receptors (NLRs) results in
immunity and a localized cell death response of infected cells. Cell death activity of many …