Nucleotide signaling networks are key to facilitate alterations in gene expression, protein
function, and enzyme activity in response to diverse stimuli. Cyclic di-adenosine …

… dacA, the S. aureus gene encoding the diadenylate cyclase … Here, we report that an S.
aureus dacA mutant can be … glycine betaine–uptake system in S. aureus. Inactivation of these …

… While significant advances have been made in our understandings of the metabolism of S.
aureus in … β-lactam antibiotics remain a gold standard for the treatment of S. aureus infections. …

For a myriad of different reasons most antimicrobial peptides (AMPs) have failed to reach
clinical application. Different AMPs have different shortcomings including but not limited to …

Central metabolic pathways control virulence and antibiotic resistance, and constitute
potential targets for antibacterial drugs. In Staphylococcus aureus the role of the pentose …

A Staphylococcus aureus strain deleted for the c‐di‐AMP cyclase gene dacA is unable to
survive in rich medium unless it acquires compensatory mutations. Previously identified …

In this protocol, we describe the isolation of genomic DNA (gDNA) from Staphylococcus
aureus strains using a chloroform extraction and ethanol precipitation method. This gDNA-…

Penicillin binding protein 2a (PBP2a)-dependent resistance to β-lactam antibiotics in
methicillin-resistant Staphylococcus aureus (MRSA) is regulated by the activity of the tricarboxylic …

In this protocol, we describe the isolation of genomic DNA (gDNA) from Staphylococcus
aureus using the Promega Nuclei Lysis and Protein Precipitation solutions. Gram-positive …

We present a protocol for the generation of a gene-deletion allelic-exchange plasmid and
its recovery in Escherichia coli for the purpose of constructing an in-frame gene deletion in …