Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and
rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (…

The great majority of globally circulating pathogens go undetected, undermining patient care
and hindering outbreak preparedness and response. To enable routine surveillance and …

… If f a = 0, we required a read depth of max(1/f hc , 275) at the position in the amplicon
sequencing method; similarly, if f hc = 0 we required a read depth of max(1/f a , 100) at the position …

Zika virus (ZIKV) is causing an unprecedented epidemic linked to severe congenital abnormalities
1 , 2 . In July 2016, mosquito-borne ZIKV transmission was reported in the continental …

The CRISPR effector Cas13 could be an effective antiviral for single-stranded RNA (ssRNA)
viruses because it programmably cleaves RNAs complementary to its CRISPR RNA (crRNA)…

Recent outbreaks of viral hemorrhagic fevers (VHFs), including Ebola virus disease (EVD)
and Lassa fever (LF), highlight the urgent need for sensitive, deployable tests to diagnose …

Abstract Design of nucleic acid-based viral diagnostics typically follows heuristic rules and,
to contend with viral variation, focuses on a genome’s conserved regions. A design process …

The emergence and outbreak of SARS-CoV-2, the causative agent of COVID-19, has rapidly
become a global concern and has highlighted the need for fast, sensitive, and specific tools …

Metagenomic sequencing has the potential to transform microbial detection and
characterization, but new tools are needed to improve its sensitivity. Here we present CATCH, a …

The emergence and outbreak of SARS-CoV-2, the causative agent of COVID-19, has rapidly
become a global concern and has highlighted the need for fast, sensitive, and specific …