The recent abundance of genome sequence data has brought an urgent need for systematic
proteomics to decipher the encoded protein networks that dictate cellular function 1 . To …

Src homology (SH) regions 2 and 3 are noncatalytic domains that are conserved among a
series of cytoplasmic signaling proteins regulated by receptor protein-tyrosine kinases, …

Mapping protein–protein interactions is an invaluable tool for understanding protein function.
Here, we report the first large‐scale study of protein–protein interactions in human cells …

Phospholipase C γ 1 (PLC γ 1) and p21 ras guanosine triphosphatase (GTPase) activating
protein (GAP) bind to and are phosphorylated by activated growth factor receptors. Both PLC …

Cytoplasmic proteins that regulate signal transduction or induce cellular transformation,
including cytoplasmic protein-tyrosine kinases, p21ras GTPase-activating protein (GAP), …

The ubiquitin system regulates virtually all aspects of cellular function. We report a method
to target the myriad enzymes that govern ubiquitination of protein substrates. We used …

The p21 ras GTPase-activating protein (GAP) down-regulates p21 ras by stimulating its intrinsic
GTPase activity. GAP is found predominantly as a monomer in the cytosol of normal cells…

Amplification of the Neu (ErbB-2 or HER-2) receptor tyrosine kinase occurs in 20 to 30% of
human mammary carcinomas, correlating with a poor clinical prognosis. We have previously …

Protein-tyrosine phosphatases (PTPs), along with protein-tyrosine kinases, play key roles in
cellular signaling. All Class I PTPs contain an essential active site cysteinyl residue, which …

N6-Methyladenosine (m 6 A) accounts for approximately 0.2% to 0.6% of all adenosine in
mammalian mRNA, representing the most abundant internal mRNA modifications. m 6 A RNA …