Neurons decentralize protein synthesis from the cell body to support the active metabolism
of remote dendritic and axonal compartments. The neuronal RNA transport apparatus, …

CRISPR/Cas9-based functional genomics have transformed our ability to elucidate mammalian
cell biology. However, most previous CRISPR-based screens were conducted in cancer …

Long-distance RNA transport enables local protein synthesis at metabolically-active sites
distant from the nucleus. This process ensures an appropriate spatial organization of proteins, …

The ability of endolysosomal organelles to move within the cytoplasm is essential for the
performance of their functions. Long-range movement involves coupling of the endolysosomes …

Accurate modeling of human neuronal cell biology has been a long‐standing challenge.
However, methods to differentiate human induced pluripotent stem cells (iPSCs) to neurons …

Background Progranulin (PGRN) is a lysosomal glycoprotein implicated in various
neurodegenerative diseases, including frontotemporal dementia and neuronal ceroid lipofuscinosis. …

Proximity-based in situ labeling techniques offer a unique way to capture both stable and
transient protein–protein and protein–organelle interactions. Combining this technology with …

Pupylation is a posttranslational modification peculiar to actinobacteria wherein proteins are
covalently modified with a small protein called the prokaryotic ubiquitin-like protein (Pup). …

Fully automated proteomic pipelines have the potential to achieve deep coverage of cellular
proteomes with high throughput and scalability. However, it is important to evaluate …

Recent work on the biophysics of proteins with low complexity, intrinsically disordered
domains that have the capacity to form biological condensates has profoundly altered the …