Decades of work have aimed to genetically reprogram T cells for therapeutic purposes 1 , 2
using recombinant viral vectors, which do not target transgenes to specific genomic sites 3 , …

To study In vivo functions of the ublqultous actlnbinding protein profilin, we generated by
antlsense and gene disruption techniques Dlctyostelium mutants that lack one or both of the …

Ca 2+ -triggered exocytosis is a hallmark of neurosecretory granules, but the cellular pathway
leading to the assembly of these regulated exocytotic carriers is poorly understood. Here …

Two profilin isoforms (profilins I and II) have been purified from Dictyostelium discoideum,
using affinity chromatography on a poly(L-proline) matrix; the isoforms could be separated by …

The biogenesis of peptide hormone secretory granules involves a series of sorting, modification,
and trafficking steps that initiate in the trans-Golgi andtrans-Golgi network (TGN). To …

Reporter proteins are valuable tools to monitor promoter activities and characterize signal
transduction pathways. Many of the currently available promoter reporters have drawbacks …

The actin cytoskeleton in motile non‐muscle cells is being regulated by a large number of
actin‐binding proteins. A deeper insight into the complex nature of the dynamic …

Analyzing protein functions is a key focus in discoverybased cell biology research. Clontech’s
revolutionary new ProteoTuner systems, based on a technology developed by Dr. Thomas …

Clontech's new ProteoTuner™ system allows one to regulate the quantity of a protein of
interest present in the cell, quickly and on demand. It uses a ligand-dependent destabilization …

DNA transposons such as piggyBac and Sleeping Beauty employ a cut-and-paste
mechanism of transposition that has proven effective for numerous gene transfer and cellular …