10.7554/eLife.46314.001 Previously, we described a novel alternative to chromatin
immunoprecipitation, CUT&RUN, in which unfixed permeabilized cells are incubated with antibody, …

Background CUT&RUN is an efficient epigenome profiling method that identifies sites of
DNA binding protein enrichment genome-wide with high signal to noise and low sequencing …

Chromatin profiling at locus resolution uncovers gene regulatory features that define cell
types and developmental trajectories, but it remains challenging to map and compare different …

Histones and their posttranslational modifications influence the regulation of many DNA-dependent
processes. Although an essential role for histone-modifying enzymes in these …

Although the in vitro structural and in vivo spatial characteristics of transcription factor (TF)
binding are well defined, TF interactions with chromatin and other companion TFs during …

Cleavage Under Targets and Tagmentation (CUT&Tag) is an antibody-directed transposase
tethering strategy for in situ chromatin profiling in small samples and single cells. We …

Aggressive cancers often have activating mutations in growth-controlling oncogenes and
inactivating mutations in tumor-suppressor genes. In neuroblastoma, amplification of the …

Sophisticated gene-regulatory mechanisms probably evolved in prokaryotes billions of years
before the emergence of modern eukaryotes, which inherited the same basic enzymatic …

Poly(A) RNA ‐binding proteins (Pabs) bind with high affinity and specificity to polyadenosine
RNA . Textbook models show a nuclear Pab, PABPN1 , and a cytoplasmic Pab, PABPC , …

Prostate cancer (PC) is driven by androgen receptor (AR) activity, a master regulator of
prostate development and homeostasis. Frontline therapies for metastatic PC deprive the AR of …